Abnormal TGFbeta levels in the amniotic fluid of Down syndrome pregnancies

The status of cytokines in amniotic fluid (AF) from chromosomally abnormal pregnancy is largely undefined. TGFbeta plays a key role in fetal growth and differentiation and is responsible for the immunoregulatory activity of AF in normal pregnancy, but its status in Down syndrome (DS) pregnancies is...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2000-10, Vol.44 (4), p.205-210
Main Authors: Bromage, S J, Lang, A K, Atkinson, I, Searle, R F
Format: Article
Language:English
Subjects:
Amniotic Fluid - immunology
Animals
Biological Assay - methods
Case-Control Studies
Cell Line
Down Syndrome - immunology
Enzyme-Linked Immunosorbent Assay
Female
Humans
Interleukin-2 - analysis
Interleukin-2 - metabolism
Mice
Mink
Pregnancy
Transforming Growth Factor beta - analysis
Transforming Growth Factor beta - metabolism
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Summary:The status of cytokines in amniotic fluid (AF) from chromosomally abnormal pregnancy is largely undefined. TGFbeta plays a key role in fetal growth and differentiation and is responsible for the immunoregulatory activity of AF in normal pregnancy, but its status in Down syndrome (DS) pregnancies is unknown. In addition we investigated the IL-2 status of AF from DS pregnancies. Midtrimester AF from chromosomally normal (n = 25) and abnormal pregnancies with DS (n = 15) were assayed for bioactive and latent TGFbeta levels using the mink lung epithelial cell growth inhibition bioassay and for IL-2 activity by the CTLL-2 cell proliferation bioassay and by ELISA. Levels of bioactive TGFbeta (mean 4.6+/-0.6 U/mL) were significantly increased in DS AF compared with the normal samples (mean 2.8+/-0.3 U/mL, P
ISSN:1046-7408