Analysis of the Complete Genome Sequence of Acute Bee Paralysis Virus Shows That It Belongs to the Novel Group of Insect-Infecting RNA Viruses

The complete genome sequence of acute bee paralysis virus (ABPV) was determined. The 9470 nucleotide, polyadenylated RNA genome encoded two open reading frames (ORF1 and ORF2), which were separated by 184 nucleotides. The deduced amino acid sequence of the 5′ ORF1 (nucleotides 605 to 6325) showed si...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2000-11, Vol.277 (2), p.457-463
Main Authors: Govan, V.A., Leat, N., Allsopp, M., Davison, S.
Format: Article
Language:English
Subjects:
Acute bee paralysis virus
Amino Acid Sequence
Animals
Apidae
Apis mellifera
Base Sequence
Bees - virology
Capsid - genetics
Cricket paralysis-like virus
Endopeptidases - genetics
Genome, Viral
Insect Viruses - chemistry
Insect Viruses - classification
Insect Viruses - genetics
Insecta
Molecular Sequence Data
Open Reading Frames
Phylogeny
Picornaviridae - classification
RNA Helicases - genetics
RNA Replicase - genetics
Sequence Alignment
Viral Structural Proteins - genetics
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Summary:The complete genome sequence of acute bee paralysis virus (ABPV) was determined. The 9470 nucleotide, polyadenylated RNA genome encoded two open reading frames (ORF1 and ORF2), which were separated by 184 nucleotides. The deduced amino acid sequence of the 5′ ORF1 (nucleotides 605 to 6325) showed significant similarity to the RNA-dependent RNA polymerase, helicase, and protease domains of viruses from the picornavirus, comovirus, calicivirus, and sequivirus families, as well as to a novel group of insect-infecting RNA viruses. The 3′ ORF2 (nucleotides 6509–9253) was proposed as encoding a capsid polyprotein with three major structural proteins (35, 33, and 24 kDa) and a minor protein (9.4 kDa). This was confirmed by N-terminal sequence analysis of two of these proteins. The overall genome structure of ABPV showed similarities to those of Drosophila C virus, Plautia stali intestine virus, Rhopalosiphum padi virus, and Himetobi P virus, which have been classified into a novel group of picorna-like insect-infecting RNA viruses called cricket paralysis-like viruses. It is suggested that ABPV belongs to the cricket paralysis-like viruses.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2000.0616