Alkaliphilic heliobacterium Heliorestis baculata sp. nov. and emended description of the genus Heliorestis

A rod-shaped heliobacterium motile by peritrichous flagella, designated strain OS-H1, was isolated from a sample of shoreline soil of the soda lake Ostozhe (pH 9.2, total salt content 0.22%) located in the steppe of south-east Siberia. In the first few transfers, the isolate produced heat-resistant...

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Bibliographic Details
Published in:Archives of microbiology 2000-10, Vol.174 (4), p.283-291
Main Authors: Bryantseva, I A, Gorlenko, V M, Kompantseva, E I, Tourova, T P, Kuznetsov, B B, Osipov, G A
Format: Article
Language:English
Subjects:
Bacteria - chemistry
Bacteria - classification
Bacteria - genetics
Bacterial Physiological Phenomena
Bacteriochlorophylls - metabolism
Carotenoids - metabolism
Culture Media
DNA, Ribosomal - analysis
DNA, Ribosomal - genetics
Fatty Acids - analysis
Fresh Water - microbiology
Genes, rRNA - genetics
Heliorestis baculata
Hydrogen-Ion Concentration
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Soil Microbiology
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Summary:A rod-shaped heliobacterium motile by peritrichous flagella, designated strain OS-H1, was isolated from a sample of shoreline soil of the soda lake Ostozhe (pH 9.2, total salt content 0.22%) located in the steppe of south-east Siberia. In the first few transfers, the isolate produced heat-resistant endospores. Like other heliobacteria, strain OS-H1 contained bacteriochlorophyll g and lacked intracytoplasmic membranes. The new isolate was a strict anaerobe and photoheterotroph. In the light and in the presence of organic compounds, strain OS-H1 oxidized sulfide to elemental sulfur and polysulfides, but was not capable of photoautotrophic growth. The isolate was an obligate alkaliphile able to grow at pH 8-10.2. The best growth was observed at pH 8.5-9.5, a temperature of 30 degrees C and at 5-10 g sodium carbonate l(-1). Biotin was required as a growth factor. The G+C content of strain OS-H1 was 45.0 mol%. Comparison of the 16S rRNA gene sequence to that of phototrophic bacteria showed strain OS-H1 to group within gram-positive bacteria of the family Heliobacteriaceae with the closest relationship to Heliorestis daurensis (95.6% similarity). Based on physiological, genetic and chemotaxonomic characteristics, the new heliobacterium is described as a new species of the genus Heliorestis, Heliorestis baculata.
ISSN:0302-8933
1432-072X
DOI:10.1007/s002030000204