Cloning and characterization of a Candida albicans gene homologous to fructose-1,6-bisphosphatase genes

By sequencing of the DNA adjacent to the Candida albicans SEC61 gene, an open reading frame encoding a polypeptide of 331 amino acids was found. The predicted protein showed a strong homology with the fructose-1,6-bisphosphatase [FbPase] from other organisms, and conserved regions included the catal...

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Bibliographic Details
Published in:Current microbiology 2000-12, Vol.41 (6), p.384-387
Main Authors: DE LA ROSA, José M, RUIZ, Teresa, RODRIGUEZ, Luis
Format: Article
Language:English
Subjects:
Amino acids
Biological and medical sciences
CaFBP1 gene
Candida albicans
Candida albicans - genetics
Carbon sources
Cloning
Cloning, Molecular
Fructose-Bisphosphatase - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Fungal
Genetic Complementation Test
Microbiology
Mycology
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Saccharomyces cerevisiae - genetics
SEC61 gene
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Transformation, Genetic
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Summary:By sequencing of the DNA adjacent to the Candida albicans SEC61 gene, an open reading frame encoding a polypeptide of 331 amino acids was found. The predicted protein showed a strong homology with the fructose-1,6-bisphosphatase [FbPase] from other organisms, and conserved regions included the catalytic motif found in all known FbPases. Although the cloned gene did not complement the growth failure of a Saccharomyces cerevisiae fbp1 mutant in media with gluconeogenic carbon sources, it was transcribed in the transformants in a fashion that indicates a partial repression by glucose. A similar control on the transcription of this gene and on FbPase activity was found in wild-type C. albicans, where the cloned gene (CaFBP1) was shown to be localized in a single chromosomal locus in the genome.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002840010154