Maturation of envelope-specific antibody responses to linear determinants in monkeys inoculated with attenuated SIV

We have previously used a panel of quantitative and qualitative serological assays to define a lengthy and complex maturation of envelope‐specific antibody responses in monkeys experimentally infected with attenuated simian immunodeficiency virus (SIV) that is closely associated with the temporal de...

Full description

Saved in:
Bibliographic Details
Published in:Journal of medical primatology 2000-08, Vol.29 (3-4), p.220-230
Main Authors: Cole, Kelly Stefano, Paliotti, Michael J., Murphey-Corb, Michael, Montelaro, Ronald C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Viral - blood
Antibody Formation
Antibody Specificity
gp120
gp41
HIV Envelope Protein gp120 - chemistry
HIV Envelope Protein gp120 - immunology
humoral immunity
Macaca mulatta
Membrane Glycoproteins - chemistry
Membrane Glycoproteins - immunology
Molecular Sequence Data
peptide antigen
Peptide Fragments - chemistry
Peptide Fragments - immunology
Retroviridae Proteins - chemistry
Retroviridae Proteins - immunology
rhesus macaque
SAIDS Vaccines - immunology
Simian Acquired Immunodeficiency Syndrome - immunology
Simian Immunodeficiency Virus - immunology
SIV
Time Factors
Vaccines, Attenuated - immunology
Viral Envelope Proteins
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have previously used a panel of quantitative and qualitative serological assays to define a lengthy and complex maturation of envelope‐specific antibody responses in monkeys experimentally infected with attenuated simian immunodeficiency virus (SIV) that is closely associated with the temporal development of enduring and protective immunity to experimental virus challenge. To elucidate in more detail the changes in antibody specificity associated with this maturation, we describe here ‘domain‐specific’ serological studies to characterize the evolution of antibody responses to defined linear determinants of the SIV envelope protein. The results of these studies reveal for the first time distinguishing differences in the evolution of antibody populations to distinct envelope peptide segments, as determined by measurements of antibody titer and avidity, indicating different patterns of antibody maturation to distinct linear envelope antigenic determinants. Thus, these data demonstrate the potential for domain‐specific serology to produce a high‐resolution characterization of SIV‐specific antibody responses that can be used to evaluate experimental vaccine responses and to identify potential immune correlates of protection.
ISSN:0047-2565
1600-0684
DOI:10.1034/j.1600-0684.2000.290315.x