arcA, the regulatory gene for the arginine catabolic pathway in Aspergillus nidulans

We have cloned and analysed the arcA gene which encodes a transcriptional activator necessary for the high-level expression of two genes for enzymes of the arginine catabolic pathway in Aspergillus nidulans: agaA (for arginase) and otaA (for ornithine transaminase, OTAse). Here we present complete g...

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Bibliographic Details
Published in:Molecular genetics and genomics : MGG 2001-12, Vol.266 (4), p.591-597
Main Authors: Empel, J, Sitkiewicz, I, Andrukiewicz, A, Lasocki, K, Borsuk, P, Weglenski, P
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arginase - biosynthesis
Arginase - genetics
Arginine - metabolism
Aspergillus nidulans - genetics
Base Sequence
Cloning
Cloning, Molecular
Enzymes
Fungal Proteins - genetics
Genes
Genes, Fungal
Genes, Regulator
Genomics
Molecular Sequence Data
Mutation
Ornithine-Oxo-Acid Transaminase - biosynthesis
Ornithine-Oxo-Acid Transaminase - genetics
Plasmids
Promoter Regions, Genetic
Proteins
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Trans-Activators - genetics
Transcription, Genetic
Zinc Fingers
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Summary:We have cloned and analysed the arcA gene which encodes a transcriptional activator necessary for the high-level expression of two genes for enzymes of the arginine catabolic pathway in Aspergillus nidulans: agaA (for arginase) and otaA (for ornithine transaminase, OTAse). Here we present complete genomic and cDNA sequences for, and describe the pattern of expression of, the arcA gene. This gene contains one intron and encodes a polypeptide of 600 amino acids. The deduced protein belongs to the family of Zn(2)Cys(6) fungal regulatory proteins. ARCA is the first known protein of this family that has glycine instead of the conserved proline at the fifth position in the second, six-residue, loop of the Zn cluster domain. We have established that transcription of the arcA gene is not self-regulated and does not depend on arginine. Two mutations in arcA, one gain-of-function and one loss-of-function, have been sequenced and the effects of these mutations on the expression of the agaA gene at the transcriptional level are reported.
ISSN:1617-4615
1617-4623
DOI:10.1007/s004380100575