Expression and immunolocalization of the 14-3-3 protein of Schistosoma mansoni

The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employ...

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Bibliographic Details
Published in:Parasitology 2001-06, Vol.123 (6), p.573-582
Main Authors: SCHECHTMAN, D., WINNEN, R., TARRAB-HAZDAI, R., RAM, D., SHINDER, V., GREVELDING, C. G., KUNZ, W., ARNON, R.
Format: Article
Language:English
Subjects:
14-3-3 protein
14-3-3 Proteins
Animals
Antibodies, Helminth - biosynthesis
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Blotting, Western
DNA, Helminth - genetics
Female
Females
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Immunohistochemistry
immunolocalization
Invertebrates
Male
Microscopy
Microscopy, Immunoelectron
Nemathelminthia. Plathelmintha
Parasites
Physiology. Development
Polymerase Chain Reaction
Rabbits
Reproductive system
Schistosoma mansoni
Schistosoma mansoni - genetics
Schistosoma mansoni - metabolism
Schistosoma mansoni - ultrastructure
signal transduction
Signal Transduction - physiology
Tyrosine 3-Monooxygenase - biosynthesis
Tyrosine 3-Monooxygenase - metabolism
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Summary:The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employed for the localization of the 14-3-3 protein in the parasite, by both immunohistochemistry and immunoelectron microscopy. The results demonstrate wide distribution of this protein. It was observed in the female excretory system, the nephridia as well as in the genital systems of both sexes, namely in the vitelline gland of female and in the testis of the male. It is also present in the parenchyma and muscle of both male and female worms. Immunoelectron microscopy demonstrated the presence of immunogold-labelled protein in the tegument, subtegument, muscle, parenchyma and in the female reproductive system, in both the cytoplasm and nucleus of vitelline cells, and oocytes. The possible role of the 14-3-3 protein in the genital organs is discussed.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182001008769