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Expression and immunolocalization of the 14-3-3 protein of Schistosoma mansoni
The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employ...
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Published in: | Parasitology 2001-06, Vol.123 (6), p.573-582 |
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description | The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employed for the localization of the 14-3-3 protein in the parasite, by both immunohistochemistry and immunoelectron microscopy. The results demonstrate wide distribution of this protein. It was observed in the female excretory system, the nephridia as well as in the genital systems of both sexes, namely in the vitelline gland of female and in the testis of the male. It is also present in the parenchyma and muscle of both male and female worms. Immunoelectron microscopy demonstrated the presence of immunogold-labelled protein in the tegument, subtegument, muscle, parenchyma and in the female reproductive system, in both the cytoplasm and nucleus of vitelline cells, and oocytes. The possible role of the 14-3-3 protein in the genital organs is discussed. |
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G. ; KUNZ, W. ; ARNON, R.</creator><creatorcontrib>SCHECHTMAN, D. ; WINNEN, R. ; TARRAB-HAZDAI, R. ; RAM, D. ; SHINDER, V. ; GREVELDING, C. G. ; KUNZ, W. ; ARNON, R.</creatorcontrib><description>The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employed for the localization of the 14-3-3 protein in the parasite, by both immunohistochemistry and immunoelectron microscopy. The results demonstrate wide distribution of this protein. It was observed in the female excretory system, the nephridia as well as in the genital systems of both sexes, namely in the vitelline gland of female and in the testis of the male. It is also present in the parenchyma and muscle of both male and female worms. Immunoelectron microscopy demonstrated the presence of immunogold-labelled protein in the tegument, subtegument, muscle, parenchyma and in the female reproductive system, in both the cytoplasm and nucleus of vitelline cells, and oocytes. The possible role of the 14-3-3 protein in the genital organs is discussed.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182001008769</identifier><identifier>PMID: 11814044</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>14-3-3 protein ; 14-3-3 Proteins ; Animals ; Antibodies, Helminth - biosynthesis ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Blotting, Western ; DNA, Helminth - genetics ; Female ; Females ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Immunohistochemistry ; immunolocalization ; Invertebrates ; Male ; Microscopy ; Microscopy, Immunoelectron ; Nemathelminthia. Plathelmintha ; Parasites ; Physiology. Development ; Polymerase Chain Reaction ; Rabbits ; Reproductive system ; Schistosoma mansoni ; Schistosoma mansoni - genetics ; Schistosoma mansoni - metabolism ; Schistosoma mansoni - ultrastructure ; signal transduction ; Signal Transduction - physiology ; Tyrosine 3-Monooxygenase - biosynthesis ; Tyrosine 3-Monooxygenase - metabolism</subject><ispartof>Parasitology, 2001-06, Vol.123 (6), p.573-582</ispartof><rights>2001 Cambridge University Press</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-b58aa2712358cc74a319ef5f171f13de8111c3eb686c4c5de618b21a557d5de63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182001008769/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,72731</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13414218$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11814044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCHECHTMAN, D.</creatorcontrib><creatorcontrib>WINNEN, R.</creatorcontrib><creatorcontrib>TARRAB-HAZDAI, R.</creatorcontrib><creatorcontrib>RAM, D.</creatorcontrib><creatorcontrib>SHINDER, V.</creatorcontrib><creatorcontrib>GREVELDING, C. G.</creatorcontrib><creatorcontrib>KUNZ, W.</creatorcontrib><creatorcontrib>ARNON, R.</creatorcontrib><title>Expression and immunolocalization of the 14-3-3 protein of Schistosoma mansoni</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employed for the localization of the 14-3-3 protein in the parasite, by both immunohistochemistry and immunoelectron microscopy. The results demonstrate wide distribution of this protein. It was observed in the female excretory system, the nephridia as well as in the genital systems of both sexes, namely in the vitelline gland of female and in the testis of the male. It is also present in the parenchyma and muscle of both male and female worms. Immunoelectron microscopy demonstrated the presence of immunogold-labelled protein in the tegument, subtegument, muscle, parenchyma and in the female reproductive system, in both the cytoplasm and nucleus of vitelline cells, and oocytes. The possible role of the 14-3-3 protein in the genital organs is discussed.</description><subject>14-3-3 protein</subject><subject>14-3-3 Proteins</subject><subject>Animals</subject><subject>Antibodies, Helminth - biosynthesis</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>DNA, Helminth - genetics</subject><subject>Female</subject><subject>Females</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Immunohistochemistry</subject><subject>immunolocalization</subject><subject>Invertebrates</subject><subject>Male</subject><subject>Microscopy</subject><subject>Microscopy, Immunoelectron</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Parasites</subject><subject>Physiology. Development</subject><subject>Polymerase Chain Reaction</subject><subject>Rabbits</subject><subject>Reproductive system</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosoma mansoni - metabolism</subject><subject>Schistosoma mansoni - ultrastructure</subject><subject>signal transduction</subject><subject>Signal Transduction - physiology</subject><subject>Tyrosine 3-Monooxygenase - biosynthesis</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNp1kEtP3DAUhS3UCobHD2CDokpll9bX71kiyksatULA2nIcpxiSeLATifbX12GijkTVleV7vnN9fBA6BvwFMMivdxhTAEUwBoyVFMsdtAAmlqUCAR_QYpLLSd9D-yk9YYwFFWQX7WUTMMzYAn2_eF1Hl5IPfWH6uvBdN_ahDda0_rcZpnFoiuHRFcBKWtJiHcPg_Nv0zj76NIQUOlN0pk-h94foY2Pa5I7m8wA9XF7cn1-Xqx9XN-dnq9JSDkNZcWUMkUAoV9ZKZigsXcMbkNAArZ0CAEtdJZSwzPLaCVAVAcO5rKcbPUCnm705zsvo0qA7n6xrW9O7MCYtCSMUBM7gp3fgUxhjn7NpkqsilGCVIdhANoaUomv0OvrOxF8asJ6a1v80nT0n8-Kx6ly9dczVZuDzDJiU22yi6a1PW44yYASmx8sNl7t0r391E5-1kFRyLa5utRCw-rbkl3ri6RzWdFX09U-3_dL_4_4BeQ-hhg</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>SCHECHTMAN, D.</creator><creator>WINNEN, R.</creator><creator>TARRAB-HAZDAI, R.</creator><creator>RAM, D.</creator><creator>SHINDER, V.</creator><creator>GREVELDING, C. G.</creator><creator>KUNZ, W.</creator><creator>ARNON, R.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Expression and immunolocalization of the 14-3-3 protein of Schistosoma mansoni</title><author>SCHECHTMAN, D. ; WINNEN, R. ; TARRAB-HAZDAI, R. ; RAM, D. ; SHINDER, V. ; GREVELDING, C. 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Plathelmintha</topic><topic>Parasites</topic><topic>Physiology. Development</topic><topic>Polymerase Chain Reaction</topic><topic>Rabbits</topic><topic>Reproductive system</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - genetics</topic><topic>Schistosoma mansoni - metabolism</topic><topic>Schistosoma mansoni - ultrastructure</topic><topic>signal transduction</topic><topic>Signal Transduction - physiology</topic><topic>Tyrosine 3-Monooxygenase - biosynthesis</topic><topic>Tyrosine 3-Monooxygenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SCHECHTMAN, D.</creatorcontrib><creatorcontrib>WINNEN, R.</creatorcontrib><creatorcontrib>TARRAB-HAZDAI, R.</creatorcontrib><creatorcontrib>RAM, D.</creatorcontrib><creatorcontrib>SHINDER, V.</creatorcontrib><creatorcontrib>GREVELDING, C. 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G.</au><au>KUNZ, W.</au><au>ARNON, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and immunolocalization of the 14-3-3 protein of Schistosoma mansoni</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>123</volume><issue>6</issue><spage>573</spage><epage>582</epage><pages>573-582</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employed for the localization of the 14-3-3 protein in the parasite, by both immunohistochemistry and immunoelectron microscopy. The results demonstrate wide distribution of this protein. It was observed in the female excretory system, the nephridia as well as in the genital systems of both sexes, namely in the vitelline gland of female and in the testis of the male. It is also present in the parenchyma and muscle of both male and female worms. Immunoelectron microscopy demonstrated the presence of immunogold-labelled protein in the tegument, subtegument, muscle, parenchyma and in the female reproductive system, in both the cytoplasm and nucleus of vitelline cells, and oocytes. The possible role of the 14-3-3 protein in the genital organs is discussed.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>11814044</pmid><doi>10.1017/S0031182001008769</doi><tpages>10</tpages></addata></record> |
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subjects | 14-3-3 protein 14-3-3 Proteins Animals Antibodies, Helminth - biosynthesis Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Blotting, Western DNA, Helminth - genetics Female Females Fundamental and applied biological sciences. Psychology Gene Expression Regulation Immunohistochemistry immunolocalization Invertebrates Male Microscopy Microscopy, Immunoelectron Nemathelminthia. Plathelmintha Parasites Physiology. Development Polymerase Chain Reaction Rabbits Reproductive system Schistosoma mansoni Schistosoma mansoni - genetics Schistosoma mansoni - metabolism Schistosoma mansoni - ultrastructure signal transduction Signal Transduction - physiology Tyrosine 3-Monooxygenase - biosynthesis Tyrosine 3-Monooxygenase - metabolism |
title | Expression and immunolocalization of the 14-3-3 protein of Schistosoma mansoni |
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