Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites

The development of cervical cancer is highly associated with human papillomavirus (HPV) infection. HPV integration into the genome of infected cervical cells is temporally associated with the acquisition of the malignant phenotype. A relationship between the sites of HPV integration in cervical canc...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2000-11, Vol.60 (21), p.5916-5921
Main Authors: THORLAND, Erik C, MYERS, Shannon L, PERSING, David H, SARKAR, Gobinda, MCGOVERN, Renee M, GOSTOUT, Bobbie S, SMITH, David I
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - virology
Chromosome Fragile Sites
Chromosome Fragility - genetics
Chromosomes, Artificial, Bacterial
Chromosomes, Human - genetics
Cloning, Molecular
DNA, Neoplasm - genetics
DNA, Viral - genetics
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Human papillomavirus 16
Humans
Medical sciences
Molecular Sequence Data
Papillomaviridae - classification
Papillomaviridae - genetics
Polymerase Chain Reaction
Tumors
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - virology
Virus Integration - genetics
Viruses
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Summary:The development of cervical cancer is highly associated with human papillomavirus (HPV) infection. HPV integration into the genome of infected cervical cells is temporally associated with the acquisition of the malignant phenotype. A relationship between the sites of HPV integration in cervical cancer and the position of the common fragile sites (CFSs) has been observed at the cytogenetic level. To explore this relationship at the molecular level, we used a PCR-based method to rapidly isolate cellular sequences flanking the sites of HPV16 integrations in primary cervical tumors. Human bacterial artificial chromosome clones were isolated based on these flanking sequences and used as probes for fluorescence in situ hybridization on metaphases derived from cells cultured in the presence of aphidicolin. Our data demonstrate that HPV16 integrations in cervical tumors frequently occur within CFSs at the molecular level. In addition, we have determined the precise molecular locations of the CFSs FRA6C and FRA17B.
ISSN:0008-5472
1538-7445