Liquid chromatographic–tandem mass spectrometric method for the analysis of a neurokinin-1 antagonist and its metabolite using automated solid-phase sample preparation and automated data handling and reporting

Pharmacokinetic studies play a vital role during the development of new pharmaceutical substances. Data presented demonstrate an accurate, precise and robust assay for a neurokinin-1 receptor antagonist and its metabolite with HPLC-MS-MS. Sample preparation is performed by solid-phase extraction (SP...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2000-10, Vol.748 (1), p.55-64
Main Authors: Schütze, D, Boss, B, Schmid, J
Format: Article
Language:English
Subjects:
Automation
Chromatography, High Pressure Liquid - methods
Neurokinin-1 receptor antagonist
Neurokinin-1 Receptor Antagonists
Pharmaceutical Preparations - blood
Pharmaceutical Preparations - urine
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:Pharmacokinetic studies play a vital role during the development of new pharmaceutical substances. Data presented demonstrate an accurate, precise and robust assay for a neurokinin-1 receptor antagonist and its metabolite with HPLC-MS-MS. Sample preparation is performed by solid-phase extraction (SPE) in the 96-well plate format. This process is fully automated with a Tecan Genesis™ pipetting system using its standard robotic manipulator arm (ROMA). All instruments are fully integrated in a study oriented laboratory information system (LIMS) with an Oracle database that communicates bi-directional with the analytical equipment. Finally, the results are reported by push button operation.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00317-0