Determination of nociceptin–orphanin FQ metabolites by capillary LC–MS

Nociceptin–orphanin FQ (OFQ/N) is a newly discovered peptide involved in pain transmission. The method is described to identify metabolic pathway of this neuropeptide in the spinal cord of rats using capillary size-exclusion liquid chromatography coupled to electrospray ionization mass spectrometry....

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Bibliographic Details
Published in:Brain research. Brain research protocols 2000-11, Vol.6 (1), p.40-46
Main Authors: Suder, P, Kotlińska, J, Łȩgowska, A, Smoluch, M, Höhne, G, Chervet, J.-P, Rolka, K, Silberring, J
Format: Article
Language:English
Subjects:
Animals
Capillary high-performance liquid chromatography
Chromatography, High Pressure Liquid
Male
Mass spectrometry
Metabolism
Neuropeptide
Nociceptin
Nociceptin–Orphanin FQ (OFQ/N)
Opioid Peptides - metabolism
Peptidase
Rats
Rats, Wistar
Spectrometry, Mass, Electrospray Ionization
Spinal Cord - metabolism
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Summary:Nociceptin–orphanin FQ (OFQ/N) is a newly discovered peptide involved in pain transmission. The method is described to identify metabolic pathway of this neuropeptide in the spinal cord of rats using capillary size-exclusion liquid chromatography coupled to electrospray ionization mass spectrometry. The applied technique is rapid and selective, and allows for simultaneous measurement and quantitation of several fragments in the incubation mixture.
ISSN:1385-299X
DOI:10.1016/S1385-299X(00)00035-0