A population of variants of a viroid closely related to citrus viroid-I in citrus plants

Sequencing analyses showed a population of variants consisting of 325-330 nucleotides (nt) of a viroid closely related to citrus viroid (CVd)-I in citrus plants. These variants, for which we propose the tentative acronym CVd-I-LSS (low sequence similarity), have only 82-85% sequence similarities to...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2000-01, Vol.145 (10), p.2105-2114
Main Authors: ITO, Takao, IEKI, Hiroyuki, OZAKI, Katsumi
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Citrus - virology
Fundamental and applied biological sciences. Psychology
Genetic Variation
Genetics
Microbiology
Molecular Sequence Data
Nucleic Acid Conformation
Phylogeny
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
RNA, Viral - chemistry
RNA, Viral - genetics
Systematics. Structure, properties and multiplication. Genetics
Viroids - genetics
Virology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sequencing analyses showed a population of variants consisting of 325-330 nucleotides (nt) of a viroid closely related to citrus viroid (CVd)-I in citrus plants. These variants, for which we propose the tentative acronym CVd-I-LSS (low sequence similarity), have only 82-85% sequence similarities to CVd-I variants. A phylogenetic tree showed that the CVd-I-LSS variants formed an individual cluster that was distinct from that of the CVd-I variants, but no intermediate variants were found which could continuously connect the population of CVd-I-LSS variants with that of CVd-I variants. Citrons (Citrus medica L.) inoculated with the CVd-I-LSS seemed to show not only moderate leaf bending like citrons infected with CVd-I but also slightly more severe epinasty than citrons with CVd-I. Other biological properties of CVd-I-LSS, such as the host range, will need to be determined in order to clarify whether CVd-I-LSS is a new viroid species or a distinct strain of CVd-I. Most of the nucleotide changes between the CVd-I-LSS and CVd-I variants were found at complementary positions of the upper and lower strands within the putative pathogenic, variable, and right terminal domains, as well as in the region surrounding the central conserved region of the predicted secondary structure of CVd-I-LSS.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050070042