Triplet state mechanism for electron transfer oxidation of DNA

The interaction of anthraquinone-2-sulfonate with nucleotides and DNA in acetonitrile and acetonitrile water solvent mixture have been studied using KrF laser photolysis aimed at elucidation of the reaction mechanism. Laser spectroscopy directly demonstrates that the initial species from interaction...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2000-08, Vol.57 (1), p.76-81
Main Authors: Ma, Jian-hua, Lin, Wei-zhen, Wang, Wen-feng, Han, Zhen-hui, Yao, Si-de, Lin, Nian-yun
Format: Article
Language:English
Subjects:
Acetonitriles - chemistry
Anthraquinone-2-sulfonate
Anthraquinones - chemistry
Circular Dichroism
Deoxyguanine Nucleotides - chemistry
DNA - chemistry
DNA - radiation effects
DNA cleavage
DNA oxidation
Electrochemistry
Electrons
Kinetics
Lasers
Nucleic Acid Conformation
Oxidation-Reduction
Photolysis
Spectrophotometry
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Summary:The interaction of anthraquinone-2-sulfonate with nucleotides and DNA in acetonitrile and acetonitrile water solvent mixture have been studied using KrF laser photolysis aimed at elucidation of the reaction mechanism. Laser spectroscopy directly demonstrates that the initial species from interaction of anthraquinone-2-sulfonate with nucleotides are radical cations of nucleotides and radical anion of anthraquinone-2-sulfonate. In addition, formation of ion pair from interaction of any of mucleotides with anthraquinone-2-sulfonate is synchronous with decay of triplet anthraquinone-2-sulfonate, which has provided dynamic evidence for initiation of electron transfer from DNA bases to triplet anthraquinone-2-sulfonate. Moreover, direct observation of stabilized DNA guanyl radical cation from interaction of anthraquinone-2-sulfonate with DNA has provided initial evidence for selective cleavage of DNA at guanine moiety. The solvent-separated ion pairs in acetonitrile have evidently dissociated into free ions, thereby enabling independent study of the behavior of guanyl radical cations and radical anion of anthraquinone-2-sulfonate.
ISSN:1011-1344
1873-2682
DOI:10.1016/S1011-1344(00)00081-6