Determination of centpropazine and its metabolite in rat serum by high-performance liquid chromatography and fluorescence detection

A new HPLC assay method was developed for the simultaneous assay for centpropazine (antidepressant) and its hydroxylated metabolite (II) to assess their pharmacokinetics and metabolism characteristics. Rat serum samples were extracted with ether, backwashed with n-hexane and injected onto the HPLC s...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2000-10, Vol.748 (2), p.331-335
Main Authors: Atul, Bhattaram Venkatesh, Gupta, Ram Chandra
Format: Article
Language:English
Subjects:
Analysis
Animals
Antidepressive Agents - blood
Biological and medical sciences
Calibration
Centpropazine
Chromatography, High Pressure Liquid - methods
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Piperazines
Rats
Reference Standards
Reproducibility of Results
Spectrometry, Fluorescence
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Summary:A new HPLC assay method was developed for the simultaneous assay for centpropazine (antidepressant) and its hydroxylated metabolite (II) to assess their pharmacokinetics and metabolism characteristics. Rat serum samples were extracted with ether, backwashed with n-hexane and injected onto the HPLC system, which used a C 18 column, gradient elution and fluorescence detection at 250 Ex/350 nm Em. Variations in intra- and inter-batch accuracy and precision were within acceptable limits of
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00345-5