Direct determination of amino acids and carbohydrates by high-performance capillary electrophoresis with refractometric detection

• Published in: Journal of Chromatography A 2000-10, Vol.894 (1), p.253-257
• Main Authors: Ivanov, A.R, Nazimov, I.V, Lobazov, A.P, Popkovich, G.B
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - analysis; Carbohydrates; Carbohydrates - analysis; Electrophoresis, Capillary - methods; Refractometry; Reproducibility of Results
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)00705-6

This is an initial report to propose a novel approach in high-performance capillary electrophoresis (HPCE) for the direct detection of compounds without natural absorbance in the UV and visible spectral range, such as amino acids and carbohydrates. A refractometry detector with the 2 nl cell (Applied Systems, Minsk, Belarus) was employed to identify amino acids and carbohydrates without derivatization. The first results are provided on separation of seven free amino acids in the phosphate running buffer and three free carbohydrates in the borate–sodium dodecyl sulfate running buffer and detection by refractometer. Fused capillaries of 50 or 75 μm internal diameter and separation voltage (10–23 kV) were applied. Detection limits ranged typically from 10 to 100 fmol and the response was linear over two orders of magnitude for most of the amino acids and carbohydrates. The HPCE system demonstrated good long-term stability and reproducibility with a relative standard deviation, less than 5% for the migration time ( n=10).