ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications

We have dissected the initial stages of fertilization by intracytoplasmic sperm injection of single spermatozoa into prime oocytes from fertile rhesus monkeys (Macaca mullata). DNA decondensation was delayed at the apical portion of the sperm head. It is possible that this asynchronous male DNA deco...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2000-12, Vol.15 (12), p.2610-2620
Main Authors: Ramalho-Santos, João, Sutovsky, Peter, Simerly, Calvin, Oko, Richard, Wessel, Gary M., Hewitson, Laura, Schatten, Gerald
Format: Article
Language:English
Subjects:
acrosome
Acrosome - physiology
Animals
Biological and medical sciences
Birth control
Bromodeoxyuridine - metabolism
Cell Nucleus - metabolism
Cell Nucleus - ultrastructure
Centrosome - physiology
Centrosome - ultrastructure
DNA - biosynthesis
DNA - ultrastructure
Female
fertilization
Gynecology. Andrology. Obstetrics
ICSI
Macaca mulatta
Male
Medical sciences
Microscopy, Electron
Microtubules - ultrastructure
perinuclear theca
Sperm Head - ultrastructure
Sperm Injections, Intracytoplasmic
spermatozoa
Spermatozoa - ultrastructure
Sterility. Assisted procreation
Zygote - ultrastructure
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Summary:We have dissected the initial stages of fertilization by intracytoplasmic sperm injection of single spermatozoa into prime oocytes from fertile rhesus monkeys (Macaca mullata). DNA decondensation was delayed at the apical portion of the sperm head. It is possible that this asynchronous male DNA decondensation could be related to the persistence of the sperm acrosome and perinuclear theca after injection. However, incomplete male pronuclear formation did not prevent sperm aster formation, microtubule nucleation and pronuclear apposition. In contrast, DNA synthesis was delayed in both pronuclei until the sperm chromatin fully decondensed, indicating that male pronuclear formation constitutes an important checkpoint during the first embryonic cell cycle.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/15.12.2610