Cell-dependent gag mutants of HIV-1 are crucially defective at the stage of uncoating/reverse transcription in non-permissive cells

We have previously shown that some of the human immunodeficiency virus type 1 (HIV-1) gag matrix (MA), capsid (CA), and nucleocapsid (NC) mutants display host-cell-dependent replication potential, and that they are defective at the early phase of the virus replication cycle in non-permissive cells....

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Bibliographic Details
Published in:Microbes and infection 2000-10, Vol.2 (12), p.1419-1423
Main Authors: Koh, Kyu-Bom, Miyaura, Maki, Yoshida, Akiko, Sakurai, Akiko, Fujita, Mikako, Adachi, Akio
Format: Article
Language:English
Subjects:
Biological and medical sciences
capsid
Capsid - genetics
Capsid - physiology
Capsid Proteins
Cell Line
Defective Viruses - genetics
Defective Viruses - physiology
DNA, Viral - biosynthesis
Fundamental and applied biological sciences. Psychology
Gag
gag gene
gag Gene Products, Human Immunodeficiency Virus
Gene Products, gag - genetics
Gene Products, gag - physiology
HeLa Cells
HIV Antigens - genetics
HIV Antigens - physiology
HIV Core Protein p24 - genetics
HIV Core Protein p24 - physiology
HIV-1
HIV-1 - genetics
HIV-1 - physiology
Human immunodeficiency virus 1
Humans
matrix
Microbiology
Mutation
nucleocapsid
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
reverse transcription
Transcription, Genetic
Transfection
Viral Proteins
Virion - chemistry
Virology
Virus Replication
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Summary:We have previously shown that some of the human immunodeficiency virus type 1 (HIV-1) gag matrix (MA), capsid (CA), and nucleocapsid (NC) mutants display host-cell-dependent replication potential, and that they are defective at the early phase of the virus replication cycle in non-permissive cells. To determine the defective replication stage of the cell-dependent mutants precisely, the processes of virus entry into cells and virus DNA synthesis were monitored by the highly sensitive enzyme-linked immunosorbent assay and polymerase chain reaction amplification analysis. The results obtained indicated that all the cell-dependent MA, CA and NC mutants are defective at the stage of uncoating/reverse transcription, and that a cellular factor(s) is involved in this process.
ISSN:1286-4579
1769-714X
DOI:10.1016/S1286-4579(00)01295-8