Poly(ADP-ribose) Polymerase Localizes to the Centrosomes and Chromosomes

Poly(ADP-ribose) polymerase (PARP) takes part mainly in regulation of DNA repair, thereby maintaining genomic stability in the nucleus. However, what role PARP plays in mitotic cells is not known. Centrosomes play an important role in maintaining the fidelity of chromosome distribution during cell d...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-11, Vol.278 (2), p.385-389
Main Authors: Kanai, Masayuki, Uchida, Masahiro, Hanai, Shuji, Uematsu, Naoya, Uchida, Kazuhiko, Miwa, Masanao
Format: Article
Language:English
Subjects:
aneuploidy
cell division
Cell Line
centrosome
Centrosome - enzymology
centrosomes
chromosomal instability
Chromosomes, Human - enzymology
Fluorescent Antibody Technique, Indirect
Humans
Mitosis
PARP
poly(ADP-ribose) polymerase
Poly(ADP-ribose) Polymerases - metabolism
Tumor Cells, Cultured
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Summary:Poly(ADP-ribose) polymerase (PARP) takes part mainly in regulation of DNA repair, thereby maintaining genomic stability in the nucleus. However, what role PARP plays in mitotic cells is not known. Centrosomes play an important role in maintaining the fidelity of chromosome distribution during cell division. Loss of these functions might cause chromosomal instability and aneuploidy. p53 and BRCA1 were recently found to localize to the centrosome at mitosis. We found that PARP is localized to the centrosomes and the chromosomes at cell-division phase and interphase by indirect immunofluorescence. Furthermore, by analysis of isolated centrosomes PARP protein was found to associate with the centrosomes during mitosis. These data suggest that PARP may be involved in maintenance of chromosomal stability.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.3801