Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer

We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14.4%) were detected based on K13 g...

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Bibliographic Details
Published in:Cancer letters 2000-11, Vol.160 (1), p.115-123
Main Authors: Hamakawa, Hiroyuki, Fukuzumi, Masakuni, Bao, Yang, Sumida, Tomoki, Kayahara, Hiroaki, Onishi, Akiko, Sogawa, Kenichi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cervical lymph node
Gene Expression Regulation, Neoplastic
Humans
Keratin 13 messenger ribonucleic acid
Keratins - genetics
Lymphatic Metastasis - diagnosis
Lymphatic Metastasis - genetics
Medical sciences
Micrometastasis: Oral cancer
Mouth Neoplasms - genetics
Mouth Neoplasms - pathology
Otorhinolaryngology. Stomatology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Tumor Cells, Cultured
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:We studied three keratin (K) gene candidates, K13, K19, and K20 mRNAs, for detecting micrometastases in cervical lymph nodes (LNs) by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 166 histologically metastasis-negative nodes, 24 micrometastatic LNs (14.4%) were detected based on K13 gene expression. Keratin 19 mRNA is an inadequate marker for the genetic diagnosis due to not only illegitimate gene expression from lymphatic tissue but also gene expression from the ectopic salivary gland. Keratin 20 mRNA showed low sensitivity. It is suggested that K13 mRNA may be a promising tumor marker among these keratin genes for detecting the micrometastases in cervical LNs of oral cancer.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(00)00574-7