Induction of IL-4-dependent, anaphylactic-type and IL-4-Independent, non-anaphylactic-type IgG1 antibodies is modulated by adjuvants

Adjuvants can modulate the levels of anaphylactic- and non-anaphylactic-type IgG1 antibodies produced in response to a particular antigen. Mice immunized with ovalbumin (OVA) in Al(OH)3 gel (alum) produced mostly the anaphylactic type, irrespective of the s.c. or i.p. route used, and this antibody w...

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Bibliographic Details
Published in:International immunology 2000-12, Vol.12 (12), p.1733-1740
Main Authors: Faquim-Mauro, Eliana L., Macedo, Mahasti S.
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
alum aluminium hydroxide gel
Aluminum Hydroxide - immunology
Aluminum Hydroxide - pharmacology
Anaphylaxis
Animals
CFA complete Freund's adjuvant
cytokine
Female
Freund's Adjuvant - immunology
Freund's Adjuvant - pharmacology
humoral response
IFA incomplete Freund's adjuvant
Immunoglobulin E - immunology
Immunoglobulin G - immunology
immunoglobulin G1
immunomodulation
Interferon-gamma - immunology
Interleukin-4 - deficiency
Interleukin-4 - genetics
Mice
Mice, Inbred BALB C
Mice, Knockout
OVA ovalbumin
ovalbumin
Ovalbumin - immunology
Ovalbumin - pharmacology
Passive Cutaneous Anaphylaxis
PCA passive cutaneous anaphylaxis
Rats
β-gal β-galactosidase
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Description
Summary:Adjuvants can modulate the levels of anaphylactic- and non-anaphylactic-type IgG1 antibodies produced in response to a particular antigen. Mice immunized with ovalbumin (OVA) in Al(OH)3 gel (alum) produced mostly the anaphylactic type, irrespective of the s.c. or i.p. route used, and this antibody was not detectable in IL-4–/– mice. In contrast, when OVA was injected in complete Freund's adjuvant (CFA), it induced substantial amounts of non-anaphylactic-type IgG1 in both IL-4+/+ and IL-4–/– mice, and some anaphylactic IgG1 antibody in IL-4+/+ mice only. When IFN-γ was neutralized by specific mAb in wild-type mice immunized with OVA in CFA, the anaphylactic-type IgG1 antibody increased reaching the same levels as in alum-injected mice. This result indicates that the induction of IFN-γ by the immunization with CFA down-regulates the production of IL-4-dependent, anaphylactic-type IgG1. Despite their different effects on IgG1 antibody production, both adjuvants dramatically increased the production of IgG2a in IL-4-deprived mice and did not induce any detectable IgE in these mice.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/12.12.1733