Identification of a basal promoter for the human Mr 72,000 type IV collagenase gene and enhanced expression in a highly metastatic cell line

A basal promoter for the Mr 72,000 type IV collagenase gene was specifically defined by chloramphenicol acetyltransferase assays of a nested set of 5' upstream fragments containing the promoter region. This core promoter is TACATCT and is a noncanonical TATA box that fits the TATA consensus seq...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1991-11, Vol.51 (22), p.6190-6193
Main Authors: TEMPLETON, N. S, STETLER-STEVENSON, W. G
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Dissemination
Enhancer Elements, Genetic
Humans
Medical sciences
Melanoma - enzymology
Melanoma - genetics
Microbial Collagenase - genetics
Molecular Sequence Data
Molecular Weight
Neoplasm Metastasis
Promoter Regions, Genetic
Transcription, Genetic
Tumor cell
Tumor Cells, Cultured
Tumors
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Summary:A basal promoter for the Mr 72,000 type IV collagenase gene was specifically defined by chloramphenicol acetyltransferase assays of a nested set of 5' upstream fragments containing the promoter region. This core promoter is TACATCT and is a noncanonical TATA box that fits the TATA consensus sequence. This sequence begins 26 base pairs in the upstream direction from the start site of transcription for the type IV collagenase gene. This basal promoter is active in the highly metastatic A2058 melanoma cell line. A putative enhancer was found between nucleotides -223 to -422 that produces a 7-fold increase in transcriptional activity in the A2058 melanoma cell line. The region immediately 5' of the basal promoter, upstream to position -422, contains a silencer and represses transcriptional activity in the nonmetastatic HT144 melanoma cell line. The results of this study are consistent with previous data that found high expression of Mr 72,000 type IV collagenase mRNA and enzymatic activity in the A2058 cell line, whereas low mRNA expression and type IV collagenase activity were found in the HT144 cell line.
ISSN:0008-5472
1538-7445