Characterization of CD44 Antigen during Lymphoid Ontogeny

• Published in: Immunobiology (1979) 1991-09, Vol.183 (1), p.1-11
• Main Authors: Collado, Antonia, de Andres, Antonia, Cañadas, Encarnación, Ruiz-Cabello, Francisco, Gomez, Ovidio, Pedrinaci, Susana, Garrido, Federico
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal; Antigens, Differentiation - immunology; B-Lymphocytes - chemistry; B-Lymphocytes - immunology; Biological and medical sciences; Blood Platelets - chemistry; Cell Differentiation - immunology; Cell Line, Transformed - chemistry; Erythrocytes - chemistry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Granulocytes - chemistry; Humans; Immunobiology; Immunoenzyme Techniques; Interferon-gamma - pharmacology; Liver - chemistry; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Precipitin Tests; Skin - chemistry; T-Lymphocytes - chemistry; Tissue Distribution
• ISSN: 0171-2985; 1878-3279
• DOI: 10.1016/S0171-2985(11)80181-6

We studied immunohistological and biochemical aspects of the CD44 molecule with a mAb produced in our lab: GRHL-1. The characteristic expression of this antigen in cells of B lineage was analyzed. This mAb showed identical immunohistological patterns of reactivity to other mAbs included in CD44 cluster, on a variety lymphoid and nonlymphoid human tissues, and demonstrated similar bands on SDS-PAGE of 125I labeled lymphocyte lysates. This antigen is limited to cells of mature phenotype, and disappears in proliferating B cells in the germinal centers of the lymphoid follicles. CD44 is absent in pre-B and Burkitt cell lines. PKC activation mediate in vitro differentiation of pre-B cell lines. However, it is not involved in up-regulation of CD44 antigen expression.