The tubB α-tubulin gene is essential for sexual development in Aspergillus nidulans

The filamentous fungus Aspergillus nidulans has two genes encoding alpha-tubulin, tubA and tubB. Mutational analysis of tubA has demonstrated that the tubA gene is essential for mitosis and nuclear migration. In this study we have deleted the tubB gene by replacing it with a selectable marker and ha...

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Bibliographic Details
Published in:Genes & development 1991-11, Vol.5 (11), p.2014-2023
Main Authors: KIRK, K. E, MORRIS, N. R
Format: Article
Language:English
Subjects:
Aspergillus nidulans - cytology
Aspergillus nidulans - drug effects
Aspergillus nidulans - genetics
Aspergillus nidulans - growth & development
Benomyl - pharmacology
Biological and medical sciences
Blotting, Southern
Cell Nucleus - physiology
DNA Mutational Analysis
Fundamental and applied biological sciences. Psychology
Genes, Fungal - genetics
Genes. Genome
Meiosis - genetics
Molecular and cellular biology
Molecular genetics
Multigene Family - genetics
Plasmids - genetics
Spores, Fungal - cytology
Spores, Fungal - genetics
Temperature
Transformation, Genetic - genetics
Tubulin - genetics
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Summary:The filamentous fungus Aspergillus nidulans has two genes encoding alpha-tubulin, tubA and tubB. Mutational analysis of tubA has demonstrated that the tubA gene is essential for mitosis and nuclear migration. In this study we have deleted the tubB gene by replacing it with a selectable marker and have named this new allele tubB delta. The results demonstrate that the tubB gene is not required for vegetative growth or asexual reproduction, nor is it required for the initiation or early stages of sexual differentiation. Deletion of tubB, however, completely prevents ascosporogenesis, because tubB delta strains produce no sexual spores when self-crossed. These strains produce viable ascospores when outcrossed to tubB+ strains, indicating that the tubB delta mutation is recessive. We have studied the cytology of sexual development in wild-type strains and in the tubB mutant and have observed that tubB delta. strains develop normally to the stage of ascus formation. However, only a single nuclear mass is observed in the tubB delta ascus, indicating that either the two zygotic haploid nuclei are blocked in karyogamy or that karyogamy occurs but the resulting diploid nucleus is subsequently blocked in meiosis I.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.5.11.2014