Quality of oocytes from superovulated rhesus monkeys

The parameters, serum oestradiol (E2) response, follicle size and cumulus morphology, which are commonly used to determine in-vivo oocyte maturation in human in-vitro fertilization and embryo transfer (IVF-ET) programmes, were shown to be unreliable predictors of maturation of rhesus oocytes. In two...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1991-05, Vol.6 (5), p.623-631
Main Authors: Johnson, Lorna D., Mattson, Britta A., Albertini, David F., Sehgal, Prabhat K., Becker, Robert A., Avis, Jacqueline, Biggers, John D.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chorionic Gonadotropin - pharmacology
Embryo Transfer
Estradiol - blood
Female
Fertilization in Vitro
Gonadotropins, Equine - pharmacology
Gynecology. Andrology. Obstetrics
Macaca mulatta
Male
Medical sciences
Meiosis - drug effects
Menotropins - pharmacology
oocyte maturation
Oocytes - cytology
Oocytes - drug effects
Oocytes - growth & development
Ovulation Induction
Parthenogenesis
rhesus
Spindle Apparatus - drug effects
Staining and Labeling
Superovulation
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Summary:The parameters, serum oestradiol (E2) response, follicle size and cumulus morphology, which are commonly used to determine in-vivo oocyte maturation in human in-vitro fertilization and embryo transfer (IVF-ET) programmes, were shown to be unreliable predictors of maturation of rhesus oocytes. In two groups of rhesus, one stimulated with pregnant mares' serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) and the other with human menopausal gonadotrophin (HMG) and HCG, the three parameters varied widely within and between protocols. Triple fluorochrome staining (TFS) for chromatin, microtubules and filamentous actin (f-actin) in oocytes at the time of collection and following 24 h in culture showed major differences in their maturation both in vivo and in vitro following priming with PMSG and HMG. In evaluating IVF protocols, TFS provides a valuable assay for the meiotic status of fixed oocytes of non-human primates.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a137395