Strategies for the improvement of an amperometric cholesterol biosensor based on electropolymerization in flow systems: use of charge-transfer mediators and platinization of the electrode

Different configurations based on an amperometric biosensor with cholesterol oxidase entrapped in a polypyrrole film have been developed with a view to improving the analytical properties of this biosensor. The alternatives considered involve the simultaneous entrapment of the enzyme and a charge-tr...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2000-12, Vol.24 (1), p.51-63
Main Authors: Vidal, Juan C., Garcia-Ruiz, Esperanza, Castillo, Juan R.
Format: Article
Language:English
Subjects:
Amperometric cholesterol biosensor
Analysis
Biological and medical sciences
Biosensing Techniques
Charge-transfer mediator
Cholesterol - analysis
Electrochemistry
Electrodes
Flavin nucleotides
Flow system
General pharmacology
Medical sciences
Overoxidized polypyrrole films
Pharmacology. Drug treatments
Platinization
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Different configurations based on an amperometric biosensor with cholesterol oxidase entrapped in a polypyrrole film have been developed with a view to improving the analytical properties of this biosensor. The alternatives considered involve the simultaneous entrapment of the enzyme and a charge-transfer mediator as well as previous platinization of the surface of the Pt electrode. Both artificial (a ferrocene derivative) and natural (flavin nucleotides) mediators were studied as constituents of the charge-transfer process between the enzyme and the electrode. The comparative study of these biosensors, which were prepared in situ in a continuous flow system, made it possible to determine the advantages and disadvantages of each configuration when applied to flow-injection determination of cholesterol.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(00)00395-2