Surgical Stress Induces Phospholipid Degradation in the Intestinal Brush Border Membrane

• Published in: The Journal of surgical research 2000-12, Vol.94 (2), p.178-184
• Main Authors: Prabhu, R., Anup, R., Balasubramanian, K.A.
• Format: Article
• Language: English
• Subjects: Abdomen - surgery; Adenosine Triphosphatases - metabolism; Alkaline Phosphatase - metabolism; Allopurinol - pharmacology; alpha-Glucosidases - metabolism; Animals; Arginine - pharmacology; BBMV; Biological and medical sciences; Biological Transport - drug effects; gamma-Glutamyltransferase - metabolism; Glucose - metabolism; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Leucyl Aminopeptidase - metabolism; Medical sciences; Membrane Lipids - metabolism; Microvilli - drug effects; Microvilli - metabolism; Miscellaneous; Oxidative Stress; phospholipase; Phospholipids - metabolism; Postoperative Complications; Rats; Sucrase - metabolism; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical Procedures, Operative - adverse effects; surgical stress; Xanthine Oxidase - antagonists & inhibitors
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1006/jsre.2000.6007

Background. Surgical stress can lead to translocation of bacteria from the intestine into the systemic circulation. The intestinal brush border membrane (BBM) plays an important role in defense against such invasion by luminal bacteria and endotoxin. Our earlier work has shown the development of oxidative stress in the intestine after surgical stress and since the BBM is sensitive to free radical attack, this study examined the effect of surgical stress on the structure and function of intestinal BBM. Methods. Intestinal BBM were isolated from control and after surgical stress and compared for structural and functional alterations. Surgical stress was also carried out following pretreatment with the xanthine oxidase inhibitor allopurinol or the nitric oxide donor l-arginine, to study the protection offered by these compounds. Results. Surgical stress affected intestinal BBM structure as well as function. A decrease in alkaline phosphatase activity and α-tocopherol content, accompanied by an increase in lipid peroxidation, was seen. Surgical stress induced phospholipid degradation with generation of arachidonic acid. Functional impairment with a decrease in glucose transport ability was also seen. These changes are prevented by inhibition of xanthine oxidase by allopurinol pretreatment but not by NO. Conclusion. Surgical stress in the small intestine causes structural and functional alterations in the BBM through oxidative stress. This damage could affect gut barrier integrity and generation of arachidonic acid might mediate distal organ dysfunction.