The Phosphoprotein Op18/Stathmin is Differentially Expressed in Ovarian Cancer

Abstract To identify potential prognostic indicators of ovarian cancer and identify targets for therapeutic strategies, mRNA differential display was used to analyze gene expression differences in normal, benign, and cancerous ovarian tissue. One cDNA isolated by this technique, Op18/stathmin, is a...

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Bibliographic Details
Published in:Cancer investigation 2000, Vol.18 (8), p.722-730
Main Authors: Price, Douglas K., Ball, Jennifer R., Bahrani-Mostafavi, Zahra, Vachris, Judith C., Kaufman, Jay S., Naumann, R. Wendel, Higgins, Robert V., Hall, James B.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Northern
Disease Progression
Female
Female genital diseases
Gene Expression Regulation, Neoplastic
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
Medical sciences
Microtubule Proteins
Ovarian Diseases - genetics
Ovarian Diseases - metabolism
Ovarian Neoplasms - chemistry
Ovarian Neoplasms - genetics
Ovary - chemistry
Phosphoproteins - analysis
Phosphoproteins - genetics
Predictive Value of Tests
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Neoplasm - genetics
Stathmin
Tumors
Up-Regulation
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Summary:Abstract To identify potential prognostic indicators of ovarian cancer and identify targets for therapeutic strategies, mRNA differential display was used to analyze gene expression differences in normal, benign, and cancerous ovarian tissue. One cDNA isolated by this technique, Op18/stathmin, is a highly conserved gene that is reported to have many different functions within a cell, including signal transduction, control of the cell cycle, and the regulation of microtubules. Quantitative Northern blot analysis of 12 malignant ovarian samples, 8 benign ovarian tumors, and 10 normal ovarian tissue samples demonstrated overexpresaion of Op18/stathmin mRNA in the malignant cancers. Immunohistochemistry showed an apparent overex-pression of Op18/stathmin protein level and an association with proliferating cells
ISSN:0735-7907
1532-4192
DOI:10.3109/07357900009012204