GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K +-selective, K +-sensing ion channel

Here we report on the molecular identification, guard cell expression and functional characterization of AtGORK, an A rabidopsis t haliana g uard cell o utward r ectifying K + channel. GORK represents a new member of the plant Shaker K + channel superfamily. When heterologously expressed in Xenopus...

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Bibliographic Details
Published in:FEBS letters 2000-12, Vol.486 (2), p.93-98
Main Authors: Ache, Peter, Becker, Dirk, Ivashikina, Natalya, Dietrich, Petra, Roelfsema, M.Rob G, Hedrich, Rainer
Format: Article
Language:English
Subjects:
Animals
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins
Arabidopsis thaliana
Gene Expression
Guard cell
Hydrogen-Ion Concentration
Oocytes - metabolism
Plant Proteins - classification
Plant Proteins - genetics
Plant Proteins - metabolism
Potassium - metabolism
Potassium Channels - classification
Potassium Channels - genetics
Potassium Channels - metabolism
Potassium Channels, Inwardly Rectifying
Potassium transport
Xenopus
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Summary:Here we report on the molecular identification, guard cell expression and functional characterization of AtGORK, an A rabidopsis t haliana g uard cell o utward r ectifying K + channel. GORK represents a new member of the plant Shaker K + channel superfamily. When heterologously expressed in Xenopus oocytes the gene product of GORK mediated depolarization-activated K + currents. In agreement with the delayed outward rectifier in intact guard cells and protoplasts thereof, GORK is activated in a voltage- and potassium-dependent manner. Furthermore, the single channel conductance and regulation of GORK in response to pH changes resembles the biophysical properties of the guard cell delayed outward rectifier. Thus GORK very likely represents the molecular entity for depolarization-induced potassium release from guard cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(00)02248-1