A novel tumor necrosis factor-α inhibitory protein, TIP-B1

TIP-B1, a novel TNF inhibitory protein, has been identified, purified and characterized from cytosolic extracts of TNF-treated human fibroblasts, and a partial TIP-B1 cDNA clone has been obtained. The (27 kDa p I≈4.5 TIP-B1 protein is unique based on both the sequence of three internal peptides (com...

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Bibliographic Details
Published in:International journal of immunopharmacology 2000-12, Vol.22 (12), p.1137-1142
Main Authors: Berleth, Erica S, Henn, Alicia D, Gurtoo, Hira L, Wollman, Robert, Alderfer, James L, Mihich, Enrico, Ehrke, M.Jane
Format: Article
Language:English
Subjects:
Apoptosis
Biological and medical sciences
Blotting, Western
Cell Line
Cell Membrane - metabolism
Cytosol - metabolism
Cytotoxicity
DNA, Complementary - biosynthesis
Fibroblasts
Humans
Immunomodulators
Intracellular Signaling Peptides and Proteins
Mammary
Medical sciences
Molecular Biology
Pharmacology. Drug treatments
Proteins - chemistry
Proteins - pharmacology
TIP-B1 protein
TNF
Tumor Necrosis Factor-alpha - antagonists & inhibitors
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Summary:TIP-B1, a novel TNF inhibitory protein, has been identified, purified and characterized from cytosolic extracts of TNF-treated human fibroblasts, and a partial TIP-B1 cDNA clone has been obtained. The (27 kDa p I≈4.5 TIP-B1 protein is unique based on both the sequence of three internal peptides (comprising 51 amino acids), and the nucleotide sequence of the corresponding cDNA clone. TNF-sensitive cells, when exposed to TIP-B1 prior to the addition of TNF, are completely protected from TNF-induced lysis. Thus, this TIP-B1 treatment effectively makes these cells TNF-resistant. Furthermore, TIP-B1 protects cells from apoptotic lysis induced by TNF. TIP-B1 does not interfere with the interactions between TNF and the TNF receptors based on flow cytometric analysis of the cellular binding of biotinylated TNF. These and other data indicate that TIP-B1 is not a soluble TNF receptor, nor an anti-TNF antibody, nor a protease that degrades TNF, yet TIP-B1 functions when added exogenously to cells. Thus, TIP-B1 is not one of the proteins previously reported to be involved in resistance to TNF. The fact that incubation of the newly discovered novel TIP-B1 with TNF-sensitive cells protects them from TNF-induced cell death, including TNF-mediated apoptosis, makes TIP-B1 a candidate for therapeutic modulation of TNF-induced effects.
ISSN:0192-0561
1879-3495
DOI:10.1016/S0192-0561(00)00071-0