The protective effect of flavonol quercetin against ultraviolet a induced oxidative stress in rats

• Published in: Toxicology (Amsterdam) 2000-11, Vol.154 (1), p.21-29
• Main Authors: Erden Inal, Mine, Kahraman, Ahmet
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Catalase - analysis; Cell metabolism, cell oxidation; Cell physiology; Female; Fundamental and applied biological sciences. Psychology; Glutathione - analysis; Glutathione Peroxidase - analysis; Glutathione Reductase - analysis; Lipid Peroxides - biosynthesis; Liver - enzymology; Malondialdehyde - analysis; Molecular and cellular biology; Oxidative stress; Oxidative Stress - drug effects; Quercetin; Quercetin - pharmacology; Rats; Rats, Sprague-Dawley; Superoxide Dismutase - analysis; Ultraviolet Rays - adverse effects; UVA
• ISSN: 0300-483X; 1879-3185
• DOI: 10.1016/S0300-483X(00)00268-7

Ultraviolet A (UVA) light exposed cells can induce the production of reactive oxygen species (ROS) which can damage the cellular elements. Antioxidants can interfere with the production of ROS. In this study, malondialdehyde (MDA), reduced glutathione (GSH), glutathione reductase (GSSGR), glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) levels were measured in the liver of rats exposed to UVA light in various doses. The effects of quercetin were determined as antioxidant on those parameters. Rats were divided into three groups as control, ultraviolet (UV), and ultraviolet+quercetin (UV+Q). UV and UV+Q group rats were irradiated 4 h per day with UVA light (1.25 mW/cm 2) during periods of 0, 3, 6, 9 days. Thus, on days 0, 3, 6 and 9, the rats have received 0, 54, 108, 162 W/cm 2 light, respectively. Quercetin (50 mg/kg body wt.) was administered intraperitoneally before each irradiation period in the UV+Q group rats. MDA level in the UV group increased significantly on day-9 when compared to the control group ( P