Mechanisms of Increased Susceptibility to Angiotensin II–Induced Apoptosis in Ventricular Cardiomyocytes of Spontaneously Hypertensive Rats

Previous findings have shown that hypotensive doses of losartan prevent the excess of apoptosis present in the hypertrophied left ventricle of adult spontaneously hypertensive rats (SHR). This study was designed to determine whether angiotensin II facilitates apoptosis in cardiomyocytes of adult SHR...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 2000-12, Vol.36 (6), p.1065-1071
Main Authors: Ravassa, Susana, Fortuño, María Antonia, González, Arantxa, López, Begoña, Zalba, Guillermo, Fortuño, Ana, Díez, Javier
Format: Article
Language:English
Subjects:
Angiotensin II - pharmacology
Animals
Apoptosis
Arterial hypertension. Arterial hypotension
bcl-2-Associated X Protein
Biological and medical sciences
Blood and lymphatic vessels
Blood Pressure
Cardiology. Vascular system
Caspase 3
Caspases - metabolism
Cells, Cultured
Enzyme Activation
Experimental diseases
Heart - drug effects
Heart Ventricles - drug effects
Heart Ventricles - pathology
Hypertension - complications
Hypertension - pathology
Hypertrophy, Left Ventricular - etiology
Hypertrophy, Left Ventricular - pathology
Male
Medical sciences
Myocardium - pathology
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-bcl-2 - metabolism
Rats
Rats, Inbred SHR
Rats, Inbred WKY
RNA, Messenger - metabolism
Tumor Suppressor Protein p53 - metabolism
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Summary:Previous findings have shown that hypotensive doses of losartan prevent the excess of apoptosis present in the hypertrophied left ventricle of adult spontaneously hypertensive rats (SHR). This study was designed to determine whether angiotensin II facilitates apoptosis in cardiomyocytes of adult SHR. Primary cultures of ventricular cardiomyocytes from 30-week-old normotensive Wistar-Kyoto rats (WKY) and SHR with left ventricular hypertrophy were exposed to 10 mol/L angiotensin II for 24 hours. Apoptotic cells were assessed by terminal deoxynucleotidyl transferase assay and confirmed by Annexin V detection. The expression of Bax-α, Bcl-2, p53 , and caspase-3 proteins was assessed by Western blot assays. The expression of BAX gene was assessed by Northern blot. Angiotensin II increased (P
ISSN:0194-911X
1524-4563
DOI:10.1161/01.hyp.36.6.1065