Local and systemic inoculation of DNA or protein gB1s-based vaccines induce a protective immunity against rabbit ocular HSV-1 infection

A secreted form of gB1 (gB1s), previously shown to protect rabbits against HSV-1 ocular infection when inoculated systemically, was delivered to rabbit periocular area to evaluate its vaccine efficacy upon local administration. The efficacy of local or systemic inoculation of a gB1s-DNA-based vaccin...

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Bibliographic Details
Published in:Vaccine 2000-12, Vol.19 (9), p.1225-1231
Main Authors: Caselli, Elisabetta, Balboni, Pier Giorgio, Incorvaia, Carlo, Argnani, Rafaela, Parmeggiani, Francesco, Cassai, Enzo, Manservigi, Roberto
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - biosynthesis
Biological and medical sciences
DNA immunization
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Herpesvirus 1, Human - immunology
Herpesvirus Vaccines - immunology
HSV-1 vaccine
Keratitis, Herpetic - prevention & control
Male
Microbiology
Ocular infection
Rabbits
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Viral Envelope Proteins - immunology
Virology
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Summary:A secreted form of gB1 (gB1s), previously shown to protect rabbits against HSV-1 ocular infection when inoculated systemically, was delivered to rabbit periocular area to evaluate its vaccine efficacy upon local administration. The efficacy of local or systemic inoculation of a gB1s-DNA-based vaccine in the rabbit model of ocular HSV-1 infection was assessed in parallel flow. Rabbits received four inoculations of the different immunogens, then immune responses and clinical symptoms were evaluated. Both the local protein and the systemic DNA administration elicited a neutralizing antibody response, reduced ocular symptoms with respect to controls ( P
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(00)00242-5