Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screen...

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Bibliographic Details
Published in:FEBS letters 2000-12, Vol.487 (2), p.267-271
Main Authors: Canfield, Ann E., Doherty, Mary Jo, Kelly, Victoria, Newman, Bill, Farrington, Claire, Grant, Michael E., Boot-Handford, Raymond P.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies
Calcification
Calcification, Physiologic
Calcium-Binding Proteins - analysis
Calcium-Binding Proteins - genetics
Calcium-Binding Proteins - metabolism
Cattle
Cell Differentiation
Cells, Cultured
Extracellular Matrix Proteins
Gene Library
Matrix Gla Protein
Molecular Sequence Data
Peptide Fragments - chemistry
Peptide Fragments - immunology
Pericyte
Pericytes - cytology
Pericytes - physiology
Retinal Vessels - cytology
Retinal Vessels - physiology
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Summary:PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(00)02363-2