Up-regulation of serine/threonine protein phosphatase type 2A regulatory subunits during methylprednisolone-induced differentiation of leukaemic HL-60 cells

Summary Serine/threonine protein phosphatase 2A (PP2A) may play a role in leukaemic cell differentiation of the HL 60 myeloid leukaemic cell‐line after methylprednisolone induction. We have investigated the specific enzyme activity and expression of catalytic and regulatory subunits of PP2A. The res...

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Bibliographic Details
Published in:Clinical and laboratory haematology 2000-10, Vol.22 (5), p.271-274
Main Authors: Aydin, H.H., Selvi, N., Saydam, G., Tobu, M., Uzunoglu, S., Uslu, R., Buyukkececi, F., Omay, S.B.
Format: Article
Language:English
Subjects:
Anti-Inflammatory Agents - pharmacology
Antineoplastic agents
Biological and medical sciences
Blotting, Western
Cell Differentiation - drug effects
Chemotherapy
Cytosol - drug effects
Cytosol - enzymology
differentiation
HL-60 Cells
Humans
leukaemia
Medical sciences
methylprednisolone
Methylprednisolone - pharmacology
Pharmacology. Drug treatments
Phosphoprotein Phosphatases - drug effects
Phosphoprotein Phosphatases - metabolism
PP2A
Protein Phosphatase 2
Protein Subunits
Time Factors
Up-Regulation
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Summary:Summary Serine/threonine protein phosphatase 2A (PP2A) may play a role in leukaemic cell differentiation of the HL 60 myeloid leukaemic cell‐line after methylprednisolone induction. We have investigated the specific enzyme activity and expression of catalytic and regulatory subunits of PP2A. The resulting specific enzyme activity and immunoblots showed an increase in enzyme activity and the expression of regulatory subunits after methylprednisolone treatment. There was no change in the expression of PP2A catalytic subunits. It is suggested that the effect of methylprednisolone on leukaemic differentiation may be the result of PP2A upregulation.
ISSN:0141-9854
1365-2257
DOI:10.1046/j.1365-2257.2000.00326.x