A preliminary evaluation of a new enzyme immunoassay to detect Chlamydia pneumoniae-specific antibodies

• Published in: Journal of microbiological methods 2000-12, Vol.43 (2), p.117-125
• Main Authors: Ossewaarde, Jacobus M, Tuuminen, Tamara, Boersma, Wim G, Sandström, Mikael, Palomäki, Pekka, Boman, Jens
• Format: Article
• Language: English
• Subjects: Adult; Antibodies; Antibodies, Bacterial - blood; Antibody Specificity; Bacterial diseases; Bacterial diseases of the respiratory system; Biological and medical sciences; Chlamydia pneumoniae; Chlamydophila Infections - diagnosis; Chlamydophila pneumoniae - immunology; Enzyme; Fluorescent Antibody Technique; Human bacterial diseases; Humans; Immunoassay; Immunoenzyme Techniques - methods; Immunoglobulin A - blood; Immunoglobulin G - blood; Immunoglobulin M - blood; Infectious diseases; Medical sciences; Reagent Kits, Diagnostic
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/S0167-7012(00)00220-7

New enzyme immunoassays (EIAs) for determination of specific IgG, IgA, and IgM antibody titers to Chlamydia pneumoniae were evaluated independently in three research laboratories. Specificity of the EIAs was enhanced by removing LPS from the chlamydial antigen. The performance of these EIAs was evaluated in comparison with the microimmunofluorescence (MIF) test using specimens from: (i) a group of adult patients with community-acquired pneumonia (CAP) previously diagnosed as having an acute chlamydial infection by the complement fixation test or the whole inclusion fluorescence test; (ii) from a group of adult patients with acute respiratory tract infections; and (iii) from a group of young children consecutively presenting with acute respiratory tract infections. The MIF test and the EIAs detected acute infections in paired serum specimens from 12 of 14 patients from the first group. Eleven of these 12 patients were positive in both tests. The MIF test detected seven acute infections in single convalescence serum specimens from eight patients. Two of these were also positive in the EIAs. Paired serum specimens from the second group of adult patients ( n=12) were collected during an epidemic of C. pneumoniae. The EIAs detected six acute infections. The MIF test detected two additional patients with acute infections. From the group of young children ( n=30), the EIAs detected two patients with acute infections. Our conclusion from this preliminary evaluation is that these EIAs could be useful for laboratory diagnosis of acute C. pneumoniae infections. Comprehensive prospective studies should provide suitable data to calculate the sensitivity, specificity, and predictive values.