Determination of perchlorate at parts-per-billion levels in plants by ion chromatography

• Published in: Journal of Chromatography A 2000-11, Vol.898 (2), p.193-199
• Main Authors: Ellington, J.Jackson, Evans, John J
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Chromatography, Liquid - methods; Economic plant physiology; Electrochemistry; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Generalities and techniques; Perchlorate; Perchlorates - analysis; Plants - chemistry; Sodium Compounds - analysis
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)00842-6

A method for the analysis of perchlorate in plants was developed, based on dry weight, and applied to the analysis of plant organs, foodstuffs, and plant products. The method reduced greatly the ionic interferences in water extracts of plant materials. The high background conductivity, due to the plant matrix, was reduced sufficiently to allow quantitation of perchlorate with little or no matrix interference. Ion chromatography (IC) on a microbore AS16 anion-exchange column and a conductivity detector was used for separation and detection of perchlorate from the ionic plant extract. The extract was heated to precipitate proteins, centrifuged, exposed to alumina, and filtered through a cartridge filled with divinylbenzene to yield a water clear extract for IC analysis, even from highly colored solutions. Heating the extract and treatment with alumina reduced substantially the ionic content of the extracts without loss of perchlorate.