Proliferating cells in histiocytic necrotizing lymphadenitis

The phenotypes of proliferating cells in histiocytic necrotizing lymphadenitis (HNL) were examined. The affected areas consisted mainly of CD 8-positive (suppressor/cytotoxic T-cells) and CD 4-positive (helper/inducer T-cells) in association with some CD 15-positive cells (monocytes). A marker of pr...

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Bibliographic Details
Published in:Virchows Archiv. B, Cell pathology Cell pathology, 1991, Vol.61 (2), p.97-100
Main Authors: OHSHIMA, K, KIKUCHI, M, SUMIYOSHI, Y, MASUDA, Y, MOHTAI, H, EGUCHI, F, TAKESHITA, M
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antibodies, Monoclonal
Antigens, CD - isolation & purification
Biological and medical sciences
CD4 Antigens - isolation & purification
CD8 Antigens - isolation & purification
Child
Female
Histiocytosis - pathology
Humans
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Ki-67 Antigen
Lewis X Antigen
Lymph Nodes - pathology
Lymphadenitis - pathology
Lymphocyte Activation - immunology
Male
Medical sciences
Nuclear Proteins - isolation & purification
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Phenotype
Time Factors
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Summary:The phenotypes of proliferating cells in histiocytic necrotizing lymphadenitis (HNL) were examined. The affected areas consisted mainly of CD 8-positive (suppressor/cytotoxic T-cells) and CD 4-positive (helper/inducer T-cells) in association with some CD 15-positive cells (monocytes). A marker of proliferating cells (Ki-67) and monoclonal antibodies for determining the phenotypes of cells (CD 4, CD 8, CD 15) in the affected areas were applied using a double-staining method. Ki-67-positive proliferating cells were mainly CD 8-positive. A few CD 4-positive cells and rare CD 15-positive cells were also Ki-67-positive. The percentage of CD 8-positive cells increased gradually over time and the ratio of CD 8-positive to proliferating cells did not decrease throughout the observation period of 6 weeks. These results suggest that the proliferation of CD 8-positive T-cells together with the accumulation of CD 4- and CD 15-positive cells is the main phenomenon occurring in HNL.
ISSN:0340-6075