Sensitivity and specificity of the test kit BAX® for screening/ E. coli O157 :H7 in ground beef : Independent laboratory study

An independent laboratory study of the BAX for Screening/E. coli O157:H7 kit was conducted at the National Food Laboratory, Inc., Dublin, CA, to complete AOAC Performance Tested Method certification. The BAX system kit was compared with the BAM culture method and a modified BAM culture method for de...

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Bibliographic Details
Published in:Journal of AOAC International 2000-11, Vol.83 (6), p.1349-1356
Main Authors: HOCHBERG, Alan M, GERHARDT, Paul N. M, CAO, Thoa K, OCASIO, Wilfredo, BARBOUR, W. Mark, MROZINSKI, Peter M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Escherichia coli O157 - chemistry
Food industries
Food microbiology
Freezing
Fundamental and applied biological sciences. Psychology
General aspects
Meat - microbiology
Meat and meat product industries
Methods of analysis, processing and quality control, regulation, standards
Reagent Kits, Diagnostic
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Summary:An independent laboratory study of the BAX for Screening/E. coli O157:H7 kit was conducted at the National Food Laboratory, Inc., Dublin, CA, to complete AOAC Performance Tested Method certification. The BAX system kit was compared with the BAM culture method and a modified BAM culture method for detection of E. coli O157:H7 in ground beef. The BAX system kit detected the target organism at levels approximately 10-fold lower than those that gave positive BAM results. This study validated product claims, and Performance Tested Method status was granted.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/83.6.1349