Cultivation-independent techniques for studying methanotroph ecology

Methane-oxidizing bacteria (methanotrophs) have attracted considerable attention over the past 30 years. They have the unique ability to use methane as sole carbon and energy source, they are found in a wide variety of environments and play a crucial role in the global methane cycle. Methanotrophs a...

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Bibliographic Details
Published in:Research in Microbiology 2000-12, Vol.151 (10), p.807-814
Main Authors: Colin Murrell, J, Radajewski, Stefan
Format: Article
Language:English
Subjects:
activity
Animal, plant and microbial ecology
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
diversity
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
Ecology
Electrophoresis, Gel, Two-Dimensional
FISH
Fundamental and applied biological sciences. Psychology
gene probes
General aspects
Genes, Bacterial
In Situ Hybridization, Fluorescence
Isotope Labeling
Methane - metabolism
methane monooxygenase
methane oxidizing bacteria
Methylococcaceae - genetics
Methylococcaceae - isolation & purification
Methylococcaceae - metabolism
Microbial ecology
Microbiology
Oxygenases - genetics
Oxygenases - metabolism
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Soil Microbiology
stable isotopes
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Summary:Methane-oxidizing bacteria (methanotrophs) have attracted considerable attention over the past 30 years. They have the unique ability to use methane as sole carbon and energy source, they are found in a wide variety of environments and play a crucial role in the global methane cycle. Methanotrophs also show considerable potential for bioremediation processes such as degradation of ground water pollutants, and for production of bulk chemicals from cheap substrates. We review here the cultivation-independent molecular biological methods that are available for the detection and characterization of methanotrophs in the natural environment.
ISSN:0923-2508
1769-7123
DOI:10.1016/S0923-2508(00)01146-3