Identification of two human glutaminase loci and tissue-specific expression of the two related genes

Glutaminolysis is initiated by either of two isoforms, K- and L-types, of the enzyme phosphate-activated glutaminase. The chromosomal localization, genomic organization, and the tissue-specific expression of the genes have been investigated in the human by using isoform-specific cDNA probes. Results...

Full description

Saved in:
Bibliographic Details
Published in:Mammalian genome 2000-12, Vol.11 (12), p.1107-1110
Main Authors: Aledo, J C, Gómez-Fabre, P M, Olalla, L, Márquez, J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
chromosome 2
Chromosomes
DNA Primers
DNA Probes
Gene Expression
Genes
Glutaminase - chemistry
Glutaminase - genetics
glutaminolysis
Humans
K-glutaminase
L-glutaminase
Molecular Sequence Data
Proteins
radiation hybrid mapping
Sequence Homology, Amino Acid
Tumor Cells, Cultured
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glutaminolysis is initiated by either of two isoforms, K- and L-types, of the enzyme phosphate-activated glutaminase. The chromosomal localization, genomic organization, and the tissue-specific expression of the genes have been investigated in the human by using isoform-specific cDNA probes. Results obtained from radiation hybrid mapping experiments assigned the K-glutaminase gene to human Chromosome (Chr) 2, and a second locus for L-glutaminase in Chr 12 was identified. Southern blot analysis with the L-cDNA probe showed hybridization to a single restriction fragment, while four to seven fragments were found to hybridize to the K-cDNA probe. The distribution of human glutaminase expression was also investigated: the L-cDNA probe detected a single band of 2.4 kb in liver, brain, and pancreas, whereas a single transcript of approximately 4.4 kb was detected in kidney, brain, heart, placenta, lung, and pancreas by using the K-cDNA probe. This work provides evidence that the human liver and kidney glutaminase isozymes are encoded by separate genes located on different chromosomes; furthermore, the expression pattern in human tissues revealed for both isoenzymes differs notably from the paradigm based upon the isoenzyme distribution in rats.
ISSN:0938-8990
1432-1777
DOI:10.1007/s003350010190