Comparison Between Lactoferrin and Subepithelial Matrix Protein Binding in Staphylococcus aureus Associated with Bovine Mastitis

Staphylococcus aureus strains (n = 100) isolated from bovine mastitis were classified according to the presence of capsular polysaccharide serotype 5 (n = 46), type 8 (n = 26), and non-5/8 (n = 28). Strains from each type were tested for protein interaction in a 125I-labeled ligand binding assay. A...

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Bibliographic Details
Published in:Journal of dairy science 1991-10, Vol.74 (10), p.3353-3359
Main Authors: Naidu, A.S., Forsgren, A., Kalfas, S., Watts, J.L., Fournier, J.M.
Format: Article
Language:English
Subjects:
Animals
capsular polysaccharide
Cattle
Extracellular Matrix Proteins - metabolism
Female
Fibrinogen - metabolism
Fibronectins - metabolism
Immunoglobulin G - metabolism
lactoferrin
Lactoferrin - metabolism
LACTOFERRINAS
LACTOFERRINE
Laminin - metabolism
MAMMITE BOVINE
MASTITIS BOVINA
Mastitis, Bovine - microbiology
matrix proteins
POLISACARIDOS
POLYHOLOSIDE
PROPIEDADES MECANICAS
PROPRIETE MECANIQUE
Protein Binding
PROTEINAS
PROTEINE
SEROTIPOS
SEROTYPE
Serotyping
Staphylococcal Infections - microbiology
Staphylococcal Infections - veterinary
STAPHYLOCOCCUS AUREUS
Staphylococcus aureus - classification
Staphylococcus aureus - metabolism
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Summary:Staphylococcus aureus strains (n = 100) isolated from bovine mastitis were classified according to the presence of capsular polysaccharide serotype 5 (n = 46), type 8 (n = 26), and non-5/8 (n = 28). Strains from each type were tested for protein interaction in a 125I-labeled ligand binding assay. A majority of type 5 and type 8 strains showed a higher degree of binding to lactoferrin, fibronectin, and IgG than the non-5/8 strains. Fibrinogen binding was low in all serotypes. Most of the type 5 and non-5/8 strains bound less than 10% laminin, whereas type 8 strains bound laminin in the 11 to 20% range. Non-5/8 strains significantly differed from type 5 in lactoferrin, fibronectin, fibrinogen, and IgG and also from type 8 in fibrinogen and IgG binding. The differences in protein binding between type 5 and type 8 were nonsignificant. The degree of lactoferrin binding in all types positively correlated with laminin binding. Lactoferrin and fibrinogen bindings were correlated in type 5 and type 8 strains. Lactoferrin and fibronectin bindings were correlated only in type 5 strains. These data suggest that bovine lactoferrin binding is common and associated with subepithelial matrix protein interactions in certain serotypes of S. aureus.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(91)78524-X