Quantification of αs1-Casein in Goat Milk from French-Alpine and Anglo-Nubian Breeds Using Reversed-Phase High Performance Liquid Chromatography

Samples of isoelectrically precipitated goat casein from the milks of French-Alpine and Anglo-Nubian breeds were separated into four components in a single run by reversed-phase HPLC. The proportion of αs1-casein thus resolved was determined quantitatively. The method uses a reversed-phase C-4 colum...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dairy science 1991-10, Vol.74 (10), p.3303-3307
Main Authors: Mora-Gutierrez, A., Kumosinski, T.F., Farrell, H.M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Breeding
caprine
casein
Caseins - analysis
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Female
Food industries
Fundamental and applied biological sciences. Psychology
Goats - genetics
Goats - physiology
high performance liquid chromatography
Lactation - genetics
Milk - analysis
Milk and cheese industries. Ice creams
reversed-phase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Samples of isoelectrically precipitated goat casein from the milks of French-Alpine and Anglo-Nubian breeds were separated into four components in a single run by reversed-phase HPLC. The proportion of αs1-casein thus resolved was determined quantitatively. The method uses a reversed-phase C-4 column and a linear gradient from 30 to 50% acetonitrile in 30min with trifluoroacetic acid constant at .1%. Sodium dodecyl sulfate-PAGE was carried out to establish the identity of the isolated components. By a comparison with previously published results for caprine and bovine milk caseins, the four peaks were identified as κ-, αs2-, αs1-, and β- casein. Quantitative variations in the chromatographically resolved αs1-casein fraction of goat milk were evident. Some individual goat milks contained high levels of αs1-casein (2.70g/L), but others contained significantly low levels (.12 g/ L). There was no statistical difference in the overall means between breeds in αs1-casein composition, but cluster analysis statistics showed three distinct categories of αs1-producers: high, medium, and low. Interestingly, 6 of 15 French-Alpine goats and only one Anglo-Nubian goat fell into the “low” producer category (.38±.2g/L). Thus, expression of the αs1- component may be genetically regulated but may not be a breed-specific trait.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(91)78516-0