Haplotype diversity of the mitochondrial DNA D-loop region in Calomys musculinus (Rodentia, Muridae) detected by PCR-RFLP

In order to contribute to knowledge of colonization patterns in the rodent Calomys musculinus, a natural reservoir of the virus producing Argentine hemorrhagic fever (AHF), we studied the haplotype diversity of the mitochondrial DNA D-loop region in five natural populations from central Argentina. D...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical genetics 2002-10, Vol.40 (9-10), p.293-302
Main Authors: González Ittig, Raúl E, Gardenal, Cristina N
Format: Article
Language:English
Subjects:
Animals
Argentine hemorrhagic fever
Colonization
D-loops
Differentiation
DNA, Mitochondrial - genetics
Enzymes
Gene flow
Genetics
Genetics, Population
Haplotypes
Local population
Mitochondrial DNA
Muridae
Natural populations
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Restriction fragment length polymorphism
Rodentia
Rodentia - genetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to contribute to knowledge of colonization patterns in the rodent Calomys musculinus, a natural reservoir of the virus producing Argentine hemorrhagic fever (AHF), we studied the haplotype diversity of the mitochondrial DNA D-loop region in five natural populations from central Argentina. Digestion with eight restriction enzymes (RsaI, MseI, Tsp509I, AluI, AciI, HaeIII, NlaIII, and AseI) revealed polymorphism in the 1300 bp fragment amplified by PCR. Twenty different composite haplotypes were detected. Hierarchical analyses indicated that almost all variation (94%) is contained within local populations. Haplotypes 1 and 2, shared by all populations, were the most frequent. Nonsignificant genetic differentiation was found among populations of the endemic and nonendemic areas of AHF: All locations sampled presented exclusive haplotypes in spite of their geographic proximity, which would support previous observations indicating restricted gene flow among C. musculinus populations.
ISSN:0006-2928
1573-4927
DOI:10.1023/A:1020250701882