Effect of pentoxifylline on sunburn cell formation in a novel supravital human skin model

Background/Aim: The aim of this study was to determine whether pentoxifylline (Pf) has an effect on sunburn cell (SBC) formation in humans. Methods: A novel supravital human skin model was used. Normal skin samples were placed on gauze in completed RPMI 1640 Medium and remained vital for 48 h. Three...

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Published in:Photodermatology, photoimmunology & photomedicine photoimmunology & photomedicine, 2000-12, Vol.16 (6), p.278-280
Main Authors: Simics, Enikö, Mahunka, Maria, Horkay, Irén, Bohnert, Elisabeth, Moll, Ingrid, Jung, Ernst G.
Format: Article
Language:English
Subjects:
Antioxidants - pharmacology
Biological and medical sciences
Cells, Cultured
human supravital model
Humans
Medical sciences
pentoxifylline
Pentoxifylline - pharmacology
Pharmacology. Drug treatments
Skin - drug effects
Skin - pathology
Skin - radiation effects
Skin, nail, hair, dermoskeleton
Sunburn - pathology
sunburn cell
sunburn cell, human supravital model
Ultraviolet Rays - adverse effects
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Summary:Background/Aim: The aim of this study was to determine whether pentoxifylline (Pf) has an effect on sunburn cell (SBC) formation in humans. Methods: A novel supravital human skin model was used. Normal skin samples were placed on gauze in completed RPMI 1640 Medium and remained vital for 48 h. Three concentrations of Pf (7.5, 15.0 and 30.0 μg/ml) were tested. After 2 h, each skin sample was irradiated with 120 mJ/cm2 of UVB. After 24‐h incubation, the samples were formalin fixed, paraffin embedded and sections were stained with haematoxylin‐eosin. Results: The mean count of SBC (10.43±1.35 (SEM)) was significantly higher in the control group (without Pf) compared with its mean count in 7.5 μg/ml Pf (5.18±0.62, P
ISSN:0905-4383
1600-0781
DOI:10.1034/j.1600-0781.2000.160608.x