Identification of endogenous gibberellins in strawberry, including the novel gibberellins GA123, GA124 and GA125

• Published in: Phytochemistry (Oxford) 2000-12, Vol.55 (8), p.887-890
• Main Authors: BLAKE, Patrick S, TAYLOR, David R, CRISP, Carol M, MANDER, Lewis N, OWEN, David J
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fruit - chemistry; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Gibberellins - chemistry; Gibberellins - isolation & purification; Growth regulators; Metabolism; Molecular Conformation; Other biological molecules; Plant growth substances; Plant physiology and development; Rosales - chemistry
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/S0031-9422(00)00237-5

Extracts of carboxylic acids from immature fruits of strawberry (Fragaria x ananassa Duch. cv. Elsanta) were analysed for gibberellins by combined gas chromatography-mass spectrometry. The following previously characterised gibberellins were identified by comparison of their mass spectra and Kovats retention indices (KRIs) with those of standards or published data: GA1, GA3, GA5, GA8, GA12, GA17, GA19, GA20, GA29, GA44, GA48, GA49, GA53, GA77, GA97, GA111 and GA112. Evidence for endogenous 1-epi GA61 (GA119) and 11alpha-OH-GA12 was also obtained. In addition, a number of putative GAs were detected. Of these, three were shown to be 12alpha-hydroxy-GA53, 12alpha-hydroxy-GA44, and 12alpha-hydroxy-GA19 by comparison with authentic compounds prepared by rational synthesis, and have been allocated the descriptors GA123, GA124 and GA125, respectively.