Neonatal and adult human heart tissues from normal subjects and patients with ischemic, dilated or hypertrophic cardiomyopathy express insulin-like growth factor binding protein-3 (IGFBP-3)

In the human fetus IGFBP-3 mRNA expression is most abundant in the skin, muscle and heart but circulating IGFBP-3 levels show age-related variations. In human heart tissues from controls and patients with either ischemic, dilated or hypertrophic cardiomyopathy (no.: 20, age-range from fetuses to eld...

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Bibliographic Details
Published in:Journal of endocrinological investigation 2000-12, Vol.23 (11), p.724-726
Main Authors: GRANATA, R, BROGLIO, F, MIGLIORINO, D, CUTRUPI, S, BALDANZI, G, SIRENO, M, FUBINI, A, GRAZIANI, A, GHIGO, E, PUCCI, A
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Blotting, Western
Cardiology. Vascular system
Cardiomegaly - metabolism
Cardiomyopathy, Dilated - metabolism
Cardiopathies: etiologic forms (general aspects and miscellaneous)
Gene Expression
Heart
Heart - embryology
Humans
Infant, Newborn
Insulin-Like Growth Factor Binding Protein 3 - genetics
Medical sciences
Myocardial Ischemia - metabolism
Myocardium - chemistry
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
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Summary:In the human fetus IGFBP-3 mRNA expression is most abundant in the skin, muscle and heart but circulating IGFBP-3 levels show age-related variations. In human heart tissues from controls and patients with either ischemic, dilated or hypertrophic cardiomyopathy (no.: 20, age-range from fetuses to elderly subjects) we determined the expression of cardiac IGFBP-3 mRNA by reverse transcriptase polymerase chain reaction (RT-PCR) and the protein by Western blotting. The same parameters were also determined in human livers. We detected IGFBP-3 mRNA in neonatal and adult as well as in fetal human heart tissues in both ventricles. Western blotting revealed the presence of IGFBP-3 in all the examined cardiac tissues. IGFBP-3 appeared to be more abundant in the heart than in the liver and in the failing hearts from patients with ischemic heart disease than in those with hypertrophic cardiomyopathy. Thus both normal and pathological human heart tissues express IGFBP-3 across lifespan and IGFBP-3 could play IGF-dependent and/or -independent actions at the myocardial level.
ISSN:0391-4097
1720-8386
DOI:10.1007/BF03345060