Additive cytotoxic effect of cisplatin and X-irradiation on human glioma cell cultures derived from biopsy-tissue

Investigation of the in vitro cytotoxic effect of X-rays, either alone or combined with cisplatin on early passage cell cultures derived from human glioblastoma multiforme biopsy tissue. Fresh tumour specimens from four patients were processed to cell cultures. The U373 glioma cell line was used as...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2000-12, Vol.126 (12), p.711-716
Main Authors: FEHLAUER, Fabian, BARTEN-VAN RIJBROEK, Angeliqué D, STALPERS, Lukas J. A, LEENSTRA, Sieger, LINDEMAN, Jan, TJAHJA, Indra, TROOST, Dirk, WOLBERS, John G, VAN DER VALK, Paul, SMINIA, Peter
Format: Article
Language:English
Subjects:
Adult
Aged
Antineoplastic agents
Antineoplastic Agents - pharmacology
Biological and medical sciences
Biopsy
Brain Neoplasms - drug therapy
Brain Neoplasms - radiotherapy
Cell Death
Cell Survival
Chemotherapy, Adjuvant
Cisplatin - pharmacology
Combined treatments (chemotherapy of immunotherapy associated with an other treatment)
Female
Glioblastoma - drug therapy
Glioblastoma - radiotherapy
Humans
Linear Models
Male
Medical sciences
Middle Aged
Pharmacology. Drug treatments
Radiation-Sensitizing Agents - pharmacology
Radiotherapy, Adjuvant
Tumor Cells, Cultured
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Summary:Investigation of the in vitro cytotoxic effect of X-rays, either alone or combined with cisplatin on early passage cell cultures derived from human glioblastoma multiforme biopsy tissue. Fresh tumour specimens from four patients were processed to cell cultures. The U373 glioma cell line was used as a reference. Early passage cell cultures were X-irradiated (0-8 Gy) either alone or in combination with cisplatin (0.5-1 microgram/ml). Cell survival was determined by either clonogenic assay or the colorimetric MTT assay. Survival curves were generated and mathematically analysed using the linear quadratic model, to obtain the radiosensitivity parameters alpha, beta, and SF2, i.e., the Surviving Fraction after 2 Gy. Two patient-derived glioma cell cultures and the U373 cell line showed rather high SF2 values of 0.61-0.72 in the clonogenic assay, indicating relative high radiation resistance. Cisplatin alone (1 microgram/ml) reduced cell survival by 10-30% (n = 4). When combined with irradiation, a clear additive cytotoxic effect of cisplatin was demonstrated by the unaltered value of the alpha-parameter for reproductive cell death. Cisplatin exerted an additive rather than radiosensitising cytotoxic effect in uncharacterised patient derived glioma cell cultures.
ISSN:0171-5216
1432-1335
DOI:10.1007/PL00008476