lal‐1: a differentially expressed novel gene during proliferation in liver regeneration and in hepatoma cells

Background: During liver regeneration, 95% of the resting hepatocytes enter in G1/S phase of the cell cycle. A number of hormones, growth factors and cytokines were identified that activate signal transduction pathways playing a primary role in hepatocyte proliferation. A wide and representative cDN...

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Published in:Genes to cells : devoted to molecular & cellular mechanisms 2002-11, Vol.7 (11), p.1183-1190
Main Authors: Della Fazia, Maria Agnese, Piobbico, Danilo, Bartoli, Daniela, Castelli, Marilena, Brancorsini, Stefano, Viola Magni, Mariapia, Servillo, Giuseppe
Format: Article
Language:English
Subjects:
Animals
Annexins - genetics
Annexins - physiology
Carcinoma, Hepatocellular - genetics
DNA, Neoplasm - genetics
Fluorescent Antibody Technique
Gene Expression Regulation, Neoplastic
Liver Neoplasms, Experimental - genetics
Liver Regeneration - genetics
Liver Regeneration - physiology
Microscopy, Fluorescence
Organ Specificity
Rats
Sequence Analysis, DNA
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Summary:Background: During liver regeneration, 95% of the resting hepatocytes enter in G1/S phase of the cell cycle. A number of hormones, growth factors and cytokines were identified that activate signal transduction pathways playing a primary role in hepatocyte proliferation. A wide and representative cDNA library containing 1.5 × 106 independent clones was constructed from regenerating liver in order to identify and characterize gene the products which play a crucial role in the first hours of the proliferative process of liver regeneration. Results: A novel gene expressed in liver regeneration was cloned by subtractive hybridization. The putative protein displays in the N′‐terminal a annexin‐like domain and an aminopeptidase domain. We named the novel gene Liver Annexin Like‐1 (lal‐1). The lal‐1 gene is modulated during liver regeneration, in hepatoma cells following physiological stimulation and after cAMP induction. Conclusion: The results indicate that lal‐1 is involved in liver regeneration and that its expression is finely regulated during proliferative process. The isolation of lal‐1 paves the way for a further characterization helping to assess lal‐1 involvement in cell function and proliferation.
ISSN:1356-9597
1365-2443
DOI:10.1046/j.1365-2443.2002.00593.x