cDNA Cloning of Radish (Raphanus sativus) Myrosinase and Tissue-Specific Expression in Root

Two cDNA clones of myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from radish (Raphanus sativus) seedlings. Both clones were identified as MB (B type myrosinase) from their sequence homology at the amino acid level to MBs cloned from other Brassicaceae species. The tissue distri...

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Bibliographic Details
Published in:Plant and cell physiology 2000-10, Vol.41 (10), p.1102-1109
Main Authors: Hara, Masakazu, Fujii, Yoko, Sasada, Yuri, Kuboi, Toru
Format: Article
Language:English
Subjects:
Amino Acid Sequence
B and C type myrosinase
Base Sequence
Brassica - enzymology
Brassica - genetics
Cloning, Molecular
DNA Primers
DNA, Complementary
Glycoside Hydrolases - chemistry
Glycoside Hydrolases - genetics
Key words: Glucosinolate — Myrosinase — Radish (Raphanus sativus) —Thioglucoside glucohydrolase (EC 3.2.3.1) —Tissue printing
MB and MC
Molecular Sequence Data
Plant Roots - enzymology
polyvinylidene difluoride
PVDF
radish myrosinase B type
Raphanus sativus
reverse transcription PCR
RMB
RT-PCR
Sequence Homology, Amino Acid
The nucleotide sequences reported in this paper have been submitted to DDBJ under accession numbers AB042186 (RMB1) and AB042187 (RMB2)
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Summary:Two cDNA clones of myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from radish (Raphanus sativus) seedlings. Both clones were identified as MB (B type myrosinase) from their sequence homology at the amino acid level to MBs cloned from other Brassicaceae species. The tissue distribution of gene expression and enzyme activity of myrosinase corresponded well to the site of glucosinolate accumulation in different tissues of radish. The myrosinase-glucosinolate system was localized in the cotyledons in the seedlings and in the peel of the root in the mature plant. Tissue printing analysis showed that myrosinase mRNA and activity were localized in the epidermis and vascular cambium that were present in the peripheral part of the root but few signals were detected in the parenchyma inside of the vascular cambium. Since the myrosinase-glucosinolate system is known to be a defense system in higher plants, the localization of the myrosinase-glucosinolate system in the peel of the root may act to protect the sink organ from the attack of herbivores or pathogens in soil.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcd034