Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR

A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concen...

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Bibliographic Details
Published in:Journal of virological methods 2002-12, Vol.106 (2), p.241-244
Main Authors: Helps, Chris, Lait, Philippa, Tasker, Séverine, Harbour, Dave
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Caliciviridae Infections - diagnosis
Caliciviridae Infections - veterinary
Caliciviridae Infections - virology
Calicivirus, Feline - genetics
Calicivirus, Feline - isolation & purification
Cat Diseases - diagnosis
Cat Diseases - virology
Cats
FCV
Fundamental and applied biological sciences. Psychology
Melting curve
Microbiology
Nucleic Acid Denaturation
Real-time PCR
Reverse Transcriptase Polymerase Chain Reaction - methods
Reverse Transcriptase Polymerase Chain Reaction - veterinary
Techniques used in virology
Virology
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Summary:A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concentrations and allows accurate quantitation of viral load.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(02)00167-2